Near-field fluorescence imaging of single molecules with a resolution in the range of 10 nm

N. Hosaka, Toshiharu Saiki

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

We demonstrate fluorescence imaging of single molecules, by near-field scanning optical microscopy (NSOM), using the illumination-collection mode of operation, with an aperture probe. Fluorescence images of single dye molecules were obtained with a spatial resolution of 15 nm, which is smaller than the diameter of the aperture (20 nm) of the probe employed. Such super-resolution may be attributable to non-radiative energy transfer from the molecules to the coated metal of the probe since the resolution obtained in the case of conventional NSOM is limited to 30-50 nm due to penetration of light into the metal.

Original languageEnglish
Pages (from-to)362-364
Number of pages3
JournalJournal of Microscopy
Volume202
Issue number2
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Optical Imaging
Near field scanning optical microscopy
Microscopy
Light Metals
near fields
Fluorescence
Imaging techniques
fluorescence
Molecules
probes
Energy Transfer
apertures
Lighting
microscopy
molecules
Coloring Agents
scanning
Metals
metals
Energy transfer

Keywords

  • Aperture probe
  • Energy transfer
  • Illumination-collection mode
  • Near-field scanning optical microscopy
  • Single molecule fluorescence
  • Spatial resolution

ASJC Scopus subject areas

  • Instrumentation

Cite this

Near-field fluorescence imaging of single molecules with a resolution in the range of 10 nm. / Hosaka, N.; Saiki, Toshiharu.

In: Journal of Microscopy, Vol. 202, No. 2, 2001, p. 362-364.

Research output: Contribution to journalArticle

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