Near-field fluorescence imaging of single molecules with a resolution in the range of 10 nm

N. Hosaka, T. Saiki

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

We demonstrate fluorescence imaging of single molecules, by near-field scanning optical microscopy (NSOM), using the illumination-collection mode of operation, with an aperture probe. Fluorescence images of single dye molecules were obtained with a spatial resolution of 15 nm, which is smaller than the diameter of the aperture (20 nm) of the probe employed. Such super-resolution may be attributable to non-radiative energy transfer from the molecules to the coated metal of the probe since the resolution obtained in the case of conventional NSOM is limited to 30-50 nm due to penetration of light into the metal.

Original languageEnglish
Pages (from-to)362-364
Number of pages3
JournalJournal of Microscopy
Volume202
Issue number2
DOIs
Publication statusPublished - 2001 May 5
Externally publishedYes

Keywords

  • Aperture probe
  • Energy transfer
  • Illumination-collection mode
  • Near-field scanning optical microscopy
  • Single molecule fluorescence
  • Spatial resolution

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

Fingerprint Dive into the research topics of 'Near-field fluorescence imaging of single molecules with a resolution in the range of 10 nm'. Together they form a unique fingerprint.

  • Cite this