Near-field fluorescence imaging of single molecules with a resolution in the range of 10 nm

N. Hosaka, T. Saiki

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    61 Citations (Scopus)

    Abstract

    We demonstrate fluorescence imaging of single molecules, by near-field scanning optical microscopy (NSOM), using the illumination-collection mode of operation, with an aperture probe. Fluorescence images of single dye molecules were obtained with a spatial resolution of 15 nm, which is smaller than the diameter of the aperture (20 nm) of the probe employed. Such super-resolution may be attributable to non-radiative energy transfer from the molecules to the coated metal of the probe since the resolution obtained in the case of conventional NSOM is limited to 30-50 nm due to penetration of light into the metal.

    Original languageEnglish
    Pages (from-to)362-364
    Number of pages3
    JournalJournal of Microscopy
    Volume202
    Issue number2
    DOIs
    Publication statusPublished - 2001 May 5

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    Keywords

    • Aperture probe
    • Energy transfer
    • Illumination-collection mode
    • Near-field scanning optical microscopy
    • Single molecule fluorescence
    • Spatial resolution

    ASJC Scopus subject areas

    • Pathology and Forensic Medicine
    • Histology

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