Near-infrared 1064 nm laser modulates migratory dendritic cells to augment the immune response to intradermal influenza vaccine

Kaitlyn Morse, Yoshifumi Kimizuka, Megan P.K. Chan, Mai Shibata, Yusuke Shimaoka, Shu Takeuchi, Benjamin Forbes, Christopher Nirschl, Binghao Li, Yang Zeng, Roderick T. Bronson, Wataru Katagiri, Ayako Shigeta, Ruxandra F. Srbulescu, Huabiao Chen, Rhea Y.Y. Tan, Kosuke Tsukada, Timothy Brauns, Jeffrey Gelfand, Ann Sluder & 4 others Joseph J. Locascio, Mark C. Poznansky, Niroshana Anandasabapathy, Satoshi Kashiwagi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Brief exposure of skin to near-infrared (NIR) laser light has been shown to augment the immune response to intradermal vaccination and thus act as an immunologic adjuvant. Although evidence indicates that the NIR laser adjuvant has the capacity to activate innate subsets including dendritic cells (DCs) in skin as conventional adjuvants do, the precise immunological mechanism by which the NIR laser adjuvant acts is largely unknown. In this study we sought to identify the cellular target of the NIR laser adjuvant by using an established mouse model of intradermal influenza vaccination and examining the alteration of responses resulting from genetic ablation of specific DC populations. We found that a continuous wave (CW) NIR laser adjuvant broadly modulates migratory DC (migDC) populations, specifically increasing and activating the Lang+ and CD11b2Lang2 subsets in skin, and that the Ab responses augmented by the CW NIR laser are dependent on DC subsets expressing CCR2 and Langerin. In comparison, a pulsed wave NIR laser adjuvant showed limited effects on the migDC subsets. Our vaccination study demonstrated that the efficacy of the CW NIR laser is significantly better than that of the pulsed wave laser, indicating that the CW NIR laser offers a desirable immunostimulatory microenvironment for migDCs. These results demonstrate the unique ability of the NIR laser adjuvant to selectively target specific migDC populations in skin depending on its parameters, and highlight the importance of optimization of laser parameters for desirable immune protection induced by an NIR laser-adjuvanted vaccine.

Original languageEnglish
Pages (from-to)1319-1332
Number of pages14
JournalJournal of Immunology
Volume199
Issue number4
DOIs
Publication statusPublished - 2017 Aug 15

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Influenza Vaccines
Dendritic Cells
Lasers
Vaccination
Skin
Population
Immunologic Adjuvants
Langerhans Cells
Human Influenza
Vaccines

ASJC Scopus subject areas

  • Immunology

Cite this

Morse, K., Kimizuka, Y., Chan, M. P. K., Shibata, M., Shimaoka, Y., Takeuchi, S., ... Kashiwagi, S. (2017). Near-infrared 1064 nm laser modulates migratory dendritic cells to augment the immune response to intradermal influenza vaccine. Journal of Immunology, 199(4), 1319-1332. https://doi.org/10.4049/jimmunol.1601873

Near-infrared 1064 nm laser modulates migratory dendritic cells to augment the immune response to intradermal influenza vaccine. / Morse, Kaitlyn; Kimizuka, Yoshifumi; Chan, Megan P.K.; Shibata, Mai; Shimaoka, Yusuke; Takeuchi, Shu; Forbes, Benjamin; Nirschl, Christopher; Li, Binghao; Zeng, Yang; Bronson, Roderick T.; Katagiri, Wataru; Shigeta, Ayako; Srbulescu, Ruxandra F.; Chen, Huabiao; Tan, Rhea Y.Y.; Tsukada, Kosuke; Brauns, Timothy; Gelfand, Jeffrey; Sluder, Ann; Locascio, Joseph J.; Poznansky, Mark C.; Anandasabapathy, Niroshana; Kashiwagi, Satoshi.

In: Journal of Immunology, Vol. 199, No. 4, 15.08.2017, p. 1319-1332.

Research output: Contribution to journalArticle

Morse, K, Kimizuka, Y, Chan, MPK, Shibata, M, Shimaoka, Y, Takeuchi, S, Forbes, B, Nirschl, C, Li, B, Zeng, Y, Bronson, RT, Katagiri, W, Shigeta, A, Srbulescu, RF, Chen, H, Tan, RYY, Tsukada, K, Brauns, T, Gelfand, J, Sluder, A, Locascio, JJ, Poznansky, MC, Anandasabapathy, N & Kashiwagi, S 2017, 'Near-infrared 1064 nm laser modulates migratory dendritic cells to augment the immune response to intradermal influenza vaccine', Journal of Immunology, vol. 199, no. 4, pp. 1319-1332. https://doi.org/10.4049/jimmunol.1601873
Morse, Kaitlyn ; Kimizuka, Yoshifumi ; Chan, Megan P.K. ; Shibata, Mai ; Shimaoka, Yusuke ; Takeuchi, Shu ; Forbes, Benjamin ; Nirschl, Christopher ; Li, Binghao ; Zeng, Yang ; Bronson, Roderick T. ; Katagiri, Wataru ; Shigeta, Ayako ; Srbulescu, Ruxandra F. ; Chen, Huabiao ; Tan, Rhea Y.Y. ; Tsukada, Kosuke ; Brauns, Timothy ; Gelfand, Jeffrey ; Sluder, Ann ; Locascio, Joseph J. ; Poznansky, Mark C. ; Anandasabapathy, Niroshana ; Kashiwagi, Satoshi. / Near-infrared 1064 nm laser modulates migratory dendritic cells to augment the immune response to intradermal influenza vaccine. In: Journal of Immunology. 2017 ; Vol. 199, No. 4. pp. 1319-1332.
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AU - Shimaoka, Yusuke

AU - Takeuchi, Shu

AU - Forbes, Benjamin

AU - Nirschl, Christopher

AU - Li, Binghao

AU - Zeng, Yang

AU - Bronson, Roderick T.

AU - Katagiri, Wataru

AU - Shigeta, Ayako

AU - Srbulescu, Ruxandra F.

AU - Chen, Huabiao

AU - Tan, Rhea Y.Y.

AU - Tsukada, Kosuke

AU - Brauns, Timothy

AU - Gelfand, Jeffrey

AU - Sluder, Ann

AU - Locascio, Joseph J.

AU - Poznansky, Mark C.

AU - Anandasabapathy, Niroshana

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