Near-Infrared Bioluminescence Imaging with a through-Bond Energy Transfer Cassette

Masahiro Abe, Ryo Nishihara, Yuma Ikeda, Takahiro Nakajima, Moritoshi Sato, Naoko Iwasawa, Shigeru Nishiyama, Ramasamy Paulmurugan, Daniel Citterio, Sung Bae Kim, Koji Suzuki

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A coelenterazine (CTZ) analogue emitting near-infrared (NIR) bioluminescence was synthesized for through-bond energy transfer (TBET)-based imaging modalities. The analogue, named Cy5-CTZ, was prepared by conjugating cyanine-5 (Cy5) dye to CTZ through an acetylene linker. This novel derivative is intrinsically fluorescent and emits NIR-shifted luminescence upon reacting with an appropriate luciferase, the Renilla luciferase. This Cy5-CTZ substrate is optically stable in physiological samples and rapidly permeabilize through the plasma membrane into the cytosolic compartment of live cells.

Original languageEnglish
Pages (from-to)1919-1923
Number of pages5
JournalChemBioChem
Volume20
Issue number15
DOIs
Publication statusPublished - 2019

Keywords

  • bioluminescence
  • dyes/pigments
  • energy transfer
  • fluorescence
  • imaging agents

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry

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    Abe, M., Nishihara, R., Ikeda, Y., Nakajima, T., Sato, M., Iwasawa, N., Nishiyama, S., Paulmurugan, R., Citterio, D., Kim, S. B., & Suzuki, K. (2019). Near-Infrared Bioluminescence Imaging with a through-Bond Energy Transfer Cassette. ChemBioChem, 20(15), 1919-1923. https://doi.org/10.1002/cbic.201900149