Near-Infrared Bioluminescence Imaging with a through-Bond Energy Transfer Cassette

Masahiro Abe, Ryo Nishihara, Yuma Ikeda, Takahiro Nakajima, Moritoshi Sato, Naoko Iwasawa, Shigeru Nishiyama, Ramasamy Paulmurugan, Daniel Citterio, Sung Bae Kim, Koji Suzuki

Research output: Contribution to journalArticle

Abstract

A coelenterazine (CTZ) analogue emitting near-infrared (NIR) bioluminescence was synthesized for through-bond energy transfer (TBET)-based imaging modalities. The analogue, named Cy5-CTZ, was prepared by conjugating cyanine-5 (Cy5) dye to CTZ through an acetylene linker. This novel derivative is intrinsically fluorescent and emits NIR-shifted luminescence upon reacting with an appropriate luciferase, the Renilla luciferase. This Cy5-CTZ substrate is optically stable in physiological samples and rapidly permeabilize through the plasma membrane into the cytosolic compartment of live cells.

Original languageEnglish
JournalChemBioChem
DOIs
Publication statusPublished - 2019 Jan 1

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Bioluminescence
Energy Transfer
Energy transfer
Infrared radiation
Imaging techniques
Renilla Luciferases
Acetylene
Cell membranes
Luminescence
Luciferases
Cell Membrane
Derivatives
coelenterazine
Substrates

Keywords

  • bioluminescence
  • dyes/pigments
  • energy transfer
  • fluorescence
  • imaging agents

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry

Cite this

Abe, M., Nishihara, R., Ikeda, Y., Nakajima, T., Sato, M., Iwasawa, N., ... Suzuki, K. (2019). Near-Infrared Bioluminescence Imaging with a through-Bond Energy Transfer Cassette. ChemBioChem. https://doi.org/10.1002/cbic.201900149

Near-Infrared Bioluminescence Imaging with a through-Bond Energy Transfer Cassette. / Abe, Masahiro; Nishihara, Ryo; Ikeda, Yuma; Nakajima, Takahiro; Sato, Moritoshi; Iwasawa, Naoko; Nishiyama, Shigeru; Paulmurugan, Ramasamy; Citterio, Daniel; Kim, Sung Bae; Suzuki, Koji.

In: ChemBioChem, 01.01.2019.

Research output: Contribution to journalArticle

Abe, M, Nishihara, R, Ikeda, Y, Nakajima, T, Sato, M, Iwasawa, N, Nishiyama, S, Paulmurugan, R, Citterio, D, Kim, SB & Suzuki, K 2019, 'Near-Infrared Bioluminescence Imaging with a through-Bond Energy Transfer Cassette', ChemBioChem. https://doi.org/10.1002/cbic.201900149
Abe, Masahiro ; Nishihara, Ryo ; Ikeda, Yuma ; Nakajima, Takahiro ; Sato, Moritoshi ; Iwasawa, Naoko ; Nishiyama, Shigeru ; Paulmurugan, Ramasamy ; Citterio, Daniel ; Kim, Sung Bae ; Suzuki, Koji. / Near-Infrared Bioluminescence Imaging with a through-Bond Energy Transfer Cassette. In: ChemBioChem. 2019.
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AU - Sato, Moritoshi

AU - Iwasawa, Naoko

AU - Nishiyama, Shigeru

AU - Paulmurugan, Ramasamy

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