TY - GEN
T1 - Near Infrared-Triggered On-Demand Adeno-Associated Virus Release from Hydrogel Microbeads for Gene Therapy
AU - Takatsuka, Shuhei
AU - Kubota, Takeshi
AU - Kurashina, Yuta
AU - Onoe, Hiroaki
N1 - Funding Information:
This work was partly supported by Grant-in Aid for Challenging Research (Expletory) (20K21828), Japan Society for the Promotion of Science (JSPS), Japan.
Publisher Copyright:
© 2022 IEEE.
PY - 2022
Y1 - 2022
N2 - This paper describes near infrared (NIR)-triggered on-demand adeno-associated virus (AAV) release from alginate hydrogel microbeads with heat transducer for gene therapy. As an AAV carrier, calcium alginate (Ca-Alg.) hydrogel microbeads were fabricated by injecting mixed pre-gel solution composed of sodium-alginate (Na-Alg.), AAV, and Fe3O4 particles (FPs: heat transducer) to CaCl2 solution to form ionically cross-linked instantly with a centrifuge-based microdroplet shooting device. The FPs generate heat due to localized surface plasmon resonance under NIR. By irradiating NIR to the microbeads, we confirmed the release of AAV and the gene transfection to HeLa cells. Our proposed NIR-triggered AAV release system would be an effective and safe controlled release system for gene therapy.
AB - This paper describes near infrared (NIR)-triggered on-demand adeno-associated virus (AAV) release from alginate hydrogel microbeads with heat transducer for gene therapy. As an AAV carrier, calcium alginate (Ca-Alg.) hydrogel microbeads were fabricated by injecting mixed pre-gel solution composed of sodium-alginate (Na-Alg.), AAV, and Fe3O4 particles (FPs: heat transducer) to CaCl2 solution to form ionically cross-linked instantly with a centrifuge-based microdroplet shooting device. The FPs generate heat due to localized surface plasmon resonance under NIR. By irradiating NIR to the microbeads, we confirmed the release of AAV and the gene transfection to HeLa cells. Our proposed NIR-triggered AAV release system would be an effective and safe controlled release system for gene therapy.
KW - Adeno-associated virus
KW - Gene therapy
KW - Hydrogel
KW - Microbeads
KW - Near infrared
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U2 - 10.1109/MEMS51670.2022.9699702
DO - 10.1109/MEMS51670.2022.9699702
M3 - Conference contribution
AN - SCOPUS:85126395120
T3 - IEEE Symposium on Mass Storage Systems and Technologies
SP - 388
EP - 391
BT - 35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022
PB - IEEE Computer Society
T2 - 35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022
Y2 - 9 January 2022 through 13 January 2022
ER -