Negative regulation of Wnt signalling by HMG2L1, a novel NLK-binding protein

Misato Yamada, Bisei Ohkawara, Naoya Ichimura, Junko Hyodo-Miura, Seiichi Urushiyama, Kyoko Shirakabe, Hiroshi Shibuya

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Background: Wnt signalling plays a critical role in many developmental processes and tumorigenesis. Wnt/β-catenin signalling induces the stabilization of cytosolic β-catenin, which interacts with TCF/LEF-1 transcription factors, thereby inducing expression of Wnt-target genes. Recent evidence suggests that a specific MAP kinase pathway involving the MAP kinase kinase kinase TAK1 and the MAP kinase NLK counteract Wnt signalling. Results: To identify NLK-interacting proteins, we performed yeast two-hybrid screening. We isolated the gene HMG2L1 and showed that injection of Xenopus HMG2L1 (xHMG2L1) mRNA into Xenopus embryos inhibited Wnt/β-catenin-induced axis duplication and expression of Wnt/β-catenin target genes. Moreover, xHMG2L1 inhibited 5-catenin-stimulated transcriptional activity in mammalian cells. Conclusions: Our findings indicate that xHMG2L1 may negatively regulate Wnt/β-catenin signalling, and that xHMG2L1 may play a role in early Xenopus development together with NLK.

Original languageEnglish
Pages (from-to)677-684
Number of pages8
JournalGenes to Cells
Volume8
Issue number8
DOIs
Publication statusPublished - 2003 Aug 1

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

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    Yamada, M., Ohkawara, B., Ichimura, N., Hyodo-Miura, J., Urushiyama, S., Shirakabe, K., & Shibuya, H. (2003). Negative regulation of Wnt signalling by HMG2L1, a novel NLK-binding protein. Genes to Cells, 8(8), 677-684. https://doi.org/10.1046/j.1365-2443.2003.00666.x