Negative regulation of Wnt signalling by HMG2L1, a novel NLK-binding protein

Misato Yamada, Bisei Ohkawara, Naoya Ichimura, Junko Hyodo-Miura, Seiichi Urushiyama, Kyoko Shirakabe, Hiroshi Shibuya

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Background: Wnt signalling plays a critical role in many developmental processes and tumorigenesis. Wnt/β-catenin signalling induces the stabilization of cytosolic β-catenin, which interacts with TCF/LEF-1 transcription factors, thereby inducing expression of Wnt-target genes. Recent evidence suggests that a specific MAP kinase pathway involving the MAP kinase kinase kinase TAK1 and the MAP kinase NLK counteract Wnt signalling. Results: To identify NLK-interacting proteins, we performed yeast two-hybrid screening. We isolated the gene HMG2L1 and showed that injection of Xenopus HMG2L1 (xHMG2L1) mRNA into Xenopus embryos inhibited Wnt/β-catenin-induced axis duplication and expression of Wnt/β-catenin target genes. Moreover, xHMG2L1 inhibited 5-catenin-stimulated transcriptional activity in mammalian cells. Conclusions: Our findings indicate that xHMG2L1 may negatively regulate Wnt/β-catenin signalling, and that xHMG2L1 may play a role in early Xenopus development together with NLK.

Original languageEnglish
Pages (from-to)677-684
Number of pages8
JournalGenes to Cells
Volume8
Issue number8
DOIs
Publication statusPublished - 2003 Aug 1

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Catenins
Xenopus
Carrier Proteins
Lymphoid Enhancer-Binding Factor 1
T Cell Transcription Factor 1
Genes
MAP Kinase Kinase Kinases
Fungal Proteins
Mitogen-Activated Protein Kinase Kinases
Carcinogenesis
Phosphotransferases
Embryonic Structures
Messenger RNA
Injections

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Yamada, M., Ohkawara, B., Ichimura, N., Hyodo-Miura, J., Urushiyama, S., Shirakabe, K., & Shibuya, H. (2003). Negative regulation of Wnt signalling by HMG2L1, a novel NLK-binding protein. Genes to Cells, 8(8), 677-684. https://doi.org/10.1046/j.1365-2443.2003.00666.x

Negative regulation of Wnt signalling by HMG2L1, a novel NLK-binding protein. / Yamada, Misato; Ohkawara, Bisei; Ichimura, Naoya; Hyodo-Miura, Junko; Urushiyama, Seiichi; Shirakabe, Kyoko; Shibuya, Hiroshi.

In: Genes to Cells, Vol. 8, No. 8, 01.08.2003, p. 677-684.

Research output: Contribution to journalArticle

Yamada, M, Ohkawara, B, Ichimura, N, Hyodo-Miura, J, Urushiyama, S, Shirakabe, K & Shibuya, H 2003, 'Negative regulation of Wnt signalling by HMG2L1, a novel NLK-binding protein', Genes to Cells, vol. 8, no. 8, pp. 677-684. https://doi.org/10.1046/j.1365-2443.2003.00666.x
Yamada M, Ohkawara B, Ichimura N, Hyodo-Miura J, Urushiyama S, Shirakabe K et al. Negative regulation of Wnt signalling by HMG2L1, a novel NLK-binding protein. Genes to Cells. 2003 Aug 1;8(8):677-684. https://doi.org/10.1046/j.1365-2443.2003.00666.x
Yamada, Misato ; Ohkawara, Bisei ; Ichimura, Naoya ; Hyodo-Miura, Junko ; Urushiyama, Seiichi ; Shirakabe, Kyoko ; Shibuya, Hiroshi. / Negative regulation of Wnt signalling by HMG2L1, a novel NLK-binding protein. In: Genes to Cells. 2003 ; Vol. 8, No. 8. pp. 677-684.
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