Nerve growth factor treatment induces high-potassium-evoked calcium-dependent acetylcholine release in cultured embryonic rat septal cells

Takeshi Suzuki, Masaru Kanagawa, Yuko Takada, Kazuko Fujimoto, Koichiro Kawashima

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The effects of dibutyryl cAMP (dbcAMP) and nerve growth factor (NGF) on acetylcholine (ACh) release from primary cultured cells of embryonic rat septum were studied. Both dbcAMP and NGF increased the activity of choline acetyltransferase and contents of intracellular and spontaneously released ACh without affecting the total protein content. High-potassium-evoked ACh release was observed in NGF-treated cells but not in untreated and dbcAMP-treated cells. These results indicate that NGF induces cholinergic maturation from induction of the ACh-synthetic enzyme to the excitation-secretion processes.

Original languageEnglish
Pages (from-to)311-314
Number of pages4
JournalBrain Research
Volume665
Issue number2
DOIs
Publication statusPublished - 1994 Dec 5

Keywords

  • Acetylcholine release
  • Acetylcholine synthesis
  • Calcium
  • Choline acetyltransferase
  • Cyclic AMP
  • Nerve growth factor
  • Septal cell

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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