Abstract
The nestin gene is expressed in many CNS stem/progenitor cells, both in the embryo and the adult, and nestin is used commonly as a marker for these cells. In this report we analyze nestin enhancer requirements in the adult CNS, using transgenic mice carrying reporter genes linked to three different nestin enhancer constructs: the genomic rat nestin gene and 5 kb of upstream nestin sequence (NesP/acZ/3), 636 bp of the rat nestin second intron (E/nestin:EGFP), and a corresponding 714 bp region from the human second intron (Nes714tk/lacZ). NesP/acZ/3 and E/nestin:EGFP mice showed reporter gene expression in stem cell-containing regions of brain and spinal cord during normal conditions. NesP/acZ/3 and E/nestin:EGFP mice showed increased expression in spinal cord after injury and NesP/acZ/3 mice displayed elevated expression in the periventricular area of the brain after injury, which was not the case for the E/nestin:EGFP mice. In contrast, no expression in adult CNS in vivo was seen in the Nes714tk/lacZ mice carrying the human enhancer, neither during normal conditions nor after injury. The Nes714 tk/lacZ mice, however, expressed the reporter gene in reactive astrocytes and CNS stem cells cultured ex vivo. Collectively, this suggests a species difference for the nestin enhancer function in adult CNS and that elements outside the second intron enhancer are required for the full injury response in vivo.
| Original language | English |
|---|---|
| Pages (from-to) | 784-794 |
| Number of pages | 11 |
| Journal | Journal of Neuroscience Research |
| Volume | 69 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2002 Sep 15 |
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Keywords
- Astrocyte
- CNS injury
- Neurogenesis
- Spinal cord
- Stem cell
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Nestin enhancer requirements for expression in normal and injured adult CNS. / Johansson, Clas B.; Lothian, Carina; Molin, Magnus; Okano, Hideyuki; Lendahl, Urban.
In: Journal of Neuroscience Research, Vol. 69, No. 6, 15.09.2002, p. 784-794.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Nestin enhancer requirements for expression in normal and injured adult CNS
AU - Johansson, Clas B.
AU - Lothian, Carina
AU - Molin, Magnus
AU - Okano, Hideyuki
AU - Lendahl, Urban
PY - 2002/9/15
Y1 - 2002/9/15
N2 - The nestin gene is expressed in many CNS stem/progenitor cells, both in the embryo and the adult, and nestin is used commonly as a marker for these cells. In this report we analyze nestin enhancer requirements in the adult CNS, using transgenic mice carrying reporter genes linked to three different nestin enhancer constructs: the genomic rat nestin gene and 5 kb of upstream nestin sequence (NesP/acZ/3), 636 bp of the rat nestin second intron (E/nestin:EGFP), and a corresponding 714 bp region from the human second intron (Nes714tk/lacZ). NesP/acZ/3 and E/nestin:EGFP mice showed reporter gene expression in stem cell-containing regions of brain and spinal cord during normal conditions. NesP/acZ/3 and E/nestin:EGFP mice showed increased expression in spinal cord after injury and NesP/acZ/3 mice displayed elevated expression in the periventricular area of the brain after injury, which was not the case for the E/nestin:EGFP mice. In contrast, no expression in adult CNS in vivo was seen in the Nes714tk/lacZ mice carrying the human enhancer, neither during normal conditions nor after injury. The Nes714 tk/lacZ mice, however, expressed the reporter gene in reactive astrocytes and CNS stem cells cultured ex vivo. Collectively, this suggests a species difference for the nestin enhancer function in adult CNS and that elements outside the second intron enhancer are required for the full injury response in vivo.
AB - The nestin gene is expressed in many CNS stem/progenitor cells, both in the embryo and the adult, and nestin is used commonly as a marker for these cells. In this report we analyze nestin enhancer requirements in the adult CNS, using transgenic mice carrying reporter genes linked to three different nestin enhancer constructs: the genomic rat nestin gene and 5 kb of upstream nestin sequence (NesP/acZ/3), 636 bp of the rat nestin second intron (E/nestin:EGFP), and a corresponding 714 bp region from the human second intron (Nes714tk/lacZ). NesP/acZ/3 and E/nestin:EGFP mice showed reporter gene expression in stem cell-containing regions of brain and spinal cord during normal conditions. NesP/acZ/3 and E/nestin:EGFP mice showed increased expression in spinal cord after injury and NesP/acZ/3 mice displayed elevated expression in the periventricular area of the brain after injury, which was not the case for the E/nestin:EGFP mice. In contrast, no expression in adult CNS in vivo was seen in the Nes714tk/lacZ mice carrying the human enhancer, neither during normal conditions nor after injury. The Nes714 tk/lacZ mice, however, expressed the reporter gene in reactive astrocytes and CNS stem cells cultured ex vivo. Collectively, this suggests a species difference for the nestin enhancer function in adult CNS and that elements outside the second intron enhancer are required for the full injury response in vivo.
KW - Astrocyte
KW - CNS injury
KW - Neurogenesis
KW - Spinal cord
KW - Stem cell
UR - http://www.scopus.com/inward/record.url?scp=0037106217&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037106217&partnerID=8YFLogxK
U2 - 10.1002/jnr.10376
DO - 10.1002/jnr.10376
M3 - Article
VL - 69
SP - 784
EP - 794
JO - Journal of Neuroscience Research
JF - Journal of Neuroscience Research
SN - 0360-4012
IS - 6
ER -