Neural stem cell mediated recovery is enhanced by Chondroitinase ABC pretreatment in chronic cervical spinal cord injury

Hidenori Suzuki, Christopher S. Ahuja, Ryan P. Salewski, Lijun Li, Kajana Satkunendrarajah, Narihito Nagoshi, Shinsuke Shibata, Michael G. Fehlings

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Traumatic spinal cord injuries (SCIs) affect millions of people worldwide; the majority of whom are in the chronic phase of their injury. Unfortunately, most current treatments target the acute/subacute injury phase as the microenvironment of chronically injured cord consists of a well-established glial scar with inhibitory chondroitin sulfate proteoglycans (CSPGs) which acts as a potent barrier to regeneration. It has been shown that CSPGs can be degraded in vivo by intrathecal Chondroitinase ABC (ChABC) to produce a more permissive environment for regeneration by endogenous cells or transplanted neural stem cells (NSCs) in the subacute phase of injury. Using a translationally-relevant clip-contusion model of cervical spinal cord injury in mice we sought to determine if ChABC pretreatment could modify the harsh chronic microenvironment to enhance subsequent regeneration by induced pluripotent stem cell-derived NSCs (iPS-NSC). Seven weeks after injury—during the chronic phase—we delivered ChABC by intrathecal osmotic pump for one week followed by intraparenchymal iPS-NSC transplant rostral and caudal to the injury epicenter. ChABC administration reduced chronic-injury scar and resulted in significantly improved iPSC-NSC survival with clear differentiation into all three neuroglial lineages. Neurons derived from transplanted cells also formed functional synapses with host circuits on patch clamp analysis. Furthermore, the combined treatment led to recovery in key functional muscle groups including forelimb grip strength and measures of forelimb/hindlimb locomotion assessed by Catwalk. This represents important proof-of-concept data that the chronically injured spinal cord can be ‘unlocked’ by ChABC pretreatment to produce a microenvironment conducive to regenerative iPS-NSC therapy.

Original languageEnglish
Article numbere0182339
JournalPLoS One
Volume12
Issue number8
DOIs
Publication statusPublished - 2017 Aug 1

Fingerprint

Enhanced recovery
Chondroitin ABC Lyase
Neural Stem Cells
Stem cells
Spinal Cord Injuries
spinal cord
stem cells
chondroitin sulfate
pretreatment
proteoglycans
Induced Pluripotent Stem Cells
forelimbs
Wounds and Injuries
Chondroitin Sulfate Proteoglycans
Regeneration
contusions
Forelimb
neuroglia
Cicatrix
synapse

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Suzuki, H., Ahuja, C. S., Salewski, R. P., Li, L., Satkunendrarajah, K., Nagoshi, N., ... Fehlings, M. G. (2017). Neural stem cell mediated recovery is enhanced by Chondroitinase ABC pretreatment in chronic cervical spinal cord injury. PLoS One, 12(8), [e0182339]. https://doi.org/10.1371/journal.pone.0182339

Neural stem cell mediated recovery is enhanced by Chondroitinase ABC pretreatment in chronic cervical spinal cord injury. / Suzuki, Hidenori; Ahuja, Christopher S.; Salewski, Ryan P.; Li, Lijun; Satkunendrarajah, Kajana; Nagoshi, Narihito; Shibata, Shinsuke; Fehlings, Michael G.

In: PLoS One, Vol. 12, No. 8, e0182339, 01.08.2017.

Research output: Contribution to journalArticle

Suzuki, Hidenori ; Ahuja, Christopher S. ; Salewski, Ryan P. ; Li, Lijun ; Satkunendrarajah, Kajana ; Nagoshi, Narihito ; Shibata, Shinsuke ; Fehlings, Michael G. / Neural stem cell mediated recovery is enhanced by Chondroitinase ABC pretreatment in chronic cervical spinal cord injury. In: PLoS One. 2017 ; Vol. 12, No. 8.
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