Neural stem/progenitor cell-laden microfibers promote transplant survival in a mouse transected spinal cord injury model

Keiko Sugai, Soraya Nishimura, Midori Kato-Negishi, Hiroaki Onoe, Shintaroh Iwanaga, Yoshiaki Toyama, Morio Matsumoto, Shoji Takeuchi, Hideyuki Okano, Masaya Nakamura

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Previous studies have demonstrated that transplantation of neural stem/progenitor cells (NS/PCs) into the lesioned spinal cord can promote functional recovery following incomplete spinal cord injury (SCI) in animal models. However, this strategy is insufficient following complete SCI because of the gap at the lesion epicenter. To obtain functional recovery in a mouse model of complete SCI, this study uses a novel collagen-based microfiber as a scaffold for engrafted NS/PCs. We hypothesized that the NS/PC-microfiber combination would facilitate lesion closure as well as transplant survival in the transected spinal cord. NS/PCs were seeded inside the novel microfibers, where they maintained their capacity to differentiate and proliferate. After transplantation, the stumps of the transected spinal cord were successfully bridged by the NS/PC-laden microfibers. Moreover, the transplanted cells migrated into the host spinal cord and differentiated into three neural lineages (astrocytes, neurons, and oligodendrocytes). However, the NS/PC-laden scaffold could not achieve a neural connection between the rostral end of the injury and the intact caudal area of the spinal cord, nor could it achieve recovery of motor function. To obtain optimal functional recovery, a microfiber design with a modified composition may be useful. Furthermore, combinatorial therapy with rehabilitation and/or medications should also be considered for practical success of biomaterial/cell transplantation-based approaches to regenerative medicine.

Original languageEnglish
Pages (from-to)1826-1838
Number of pages13
JournalJournal of Neuroscience Research
Volume93
Issue number12
DOIs
Publication statusPublished - 2015 Dec 1

Fingerprint

Neural Stem Cells
Spinal Cord Injuries
Spinal Cord
Stem Cells
Transplants
Transplantation
Regenerative Medicine
Cell Transplantation
Recovery of Function
Oligodendroglia
Biocompatible Materials
Astrocytes
Collagen
Rehabilitation
Animal Models
Neurons
Wounds and Injuries

Keywords

  • Biomaterial
  • Microfiber
  • Neural stem/progenitor cell
  • Spinal cord injury

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Neural stem/progenitor cell-laden microfibers promote transplant survival in a mouse transected spinal cord injury model. / Sugai, Keiko; Nishimura, Soraya; Kato-Negishi, Midori; Onoe, Hiroaki; Iwanaga, Shintaroh; Toyama, Yoshiaki; Matsumoto, Morio; Takeuchi, Shoji; Okano, Hideyuki; Nakamura, Masaya.

In: Journal of Neuroscience Research, Vol. 93, No. 12, 01.12.2015, p. 1826-1838.

Research output: Contribution to journalArticle

Sugai, Keiko ; Nishimura, Soraya ; Kato-Negishi, Midori ; Onoe, Hiroaki ; Iwanaga, Shintaroh ; Toyama, Yoshiaki ; Matsumoto, Morio ; Takeuchi, Shoji ; Okano, Hideyuki ; Nakamura, Masaya. / Neural stem/progenitor cell-laden microfibers promote transplant survival in a mouse transected spinal cord injury model. In: Journal of Neuroscience Research. 2015 ; Vol. 93, No. 12. pp. 1826-1838.
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