Neurite outgrowth stimulation by n-3 and n-6 PUFAs of phospholipids in apoE-containing lipoproteins secreted from glial cells

Mitsuhiro Nakato, Michinori Matsuo, Nozomu Kono, Makoto Arita, Hiroyuki Arai, Jun Ogawa, Noriyuki Kioka, Kazumitsu Ueda

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

PUFAs, which account for 25-30% of the total fatty acids in the human brain, are important for normal brain development and cognitive function. However, it remains unclear how PUFAs are delivered to neurons and exert their effects. In this study, we demonstrated that n-3 and n-6 PUFAs added to the medium are incorporated into membrane phospholipids of primary glial cells from rat cortices, and then secreted as the fatty acid moiety of phospholipids in apoE-containing lipoproteins (LpEs). Tandem mass spectrometry analysis further showed that LpEs secreted from glial cells contain a variety of metabolites of PUFAs produced in glial cells by elongation and unsaturation. LpEs are absorbed by endocytosis into neurons via LDL receptorrelated protein 1. LpE-containing n-3 and n-6 PUFAs exhibit a strong effect on neurite outgrowth of hippocampal neurons by increasing the number of branches. This study sheds light on the novel role of LpEs in the central nervous system and also a novel pathway in which PUFAs act on neurons.

Original languageEnglish
Pages (from-to)1880-1890
Number of pages11
JournalJournal of Lipid Research
Volume56
Issue number10
DOIs
Publication statusPublished - 2015 Oct 1
Externally publishedYes

Fingerprint

Apolipoproteins E
Neuroglia
Lipoproteins
Neurons
Phospholipids
Brain
Fatty Acids
Neurology
Metabolites
Tandem Mass Spectrometry
Endocytosis
Cognition
Mass spectrometry
Rats
Elongation
Central Nervous System
Membranes
Neuronal Outgrowth
Proteins

Keywords

  • Apolipoprotein E
  • Apolipoprotein E-containing lipoprotein
  • Low density lipoprotein receptor-related protein 1
  • Neuron
  • Polyunsaturated fatty acid

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Endocrinology

Cite this

Neurite outgrowth stimulation by n-3 and n-6 PUFAs of phospholipids in apoE-containing lipoproteins secreted from glial cells. / Nakato, Mitsuhiro; Matsuo, Michinori; Kono, Nozomu; Arita, Makoto; Arai, Hiroyuki; Ogawa, Jun; Kioka, Noriyuki; Ueda, Kazumitsu.

In: Journal of Lipid Research, Vol. 56, No. 10, 01.10.2015, p. 1880-1890.

Research output: Contribution to journalArticle

Nakato, Mitsuhiro ; Matsuo, Michinori ; Kono, Nozomu ; Arita, Makoto ; Arai, Hiroyuki ; Ogawa, Jun ; Kioka, Noriyuki ; Ueda, Kazumitsu. / Neurite outgrowth stimulation by n-3 and n-6 PUFAs of phospholipids in apoE-containing lipoproteins secreted from glial cells. In: Journal of Lipid Research. 2015 ; Vol. 56, No. 10. pp. 1880-1890.
@article{7b1ad26dd756414e894b1e3ad53ec7e3,
title = "Neurite outgrowth stimulation by n-3 and n-6 PUFAs of phospholipids in apoE-containing lipoproteins secreted from glial cells",
abstract = "PUFAs, which account for 25-30{\%} of the total fatty acids in the human brain, are important for normal brain development and cognitive function. However, it remains unclear how PUFAs are delivered to neurons and exert their effects. In this study, we demonstrated that n-3 and n-6 PUFAs added to the medium are incorporated into membrane phospholipids of primary glial cells from rat cortices, and then secreted as the fatty acid moiety of phospholipids in apoE-containing lipoproteins (LpEs). Tandem mass spectrometry analysis further showed that LpEs secreted from glial cells contain a variety of metabolites of PUFAs produced in glial cells by elongation and unsaturation. LpEs are absorbed by endocytosis into neurons via LDL receptorrelated protein 1. LpE-containing n-3 and n-6 PUFAs exhibit a strong effect on neurite outgrowth of hippocampal neurons by increasing the number of branches. This study sheds light on the novel role of LpEs in the central nervous system and also a novel pathway in which PUFAs act on neurons.",
keywords = "Apolipoprotein E, Apolipoprotein E-containing lipoprotein, Low density lipoprotein receptor-related protein 1, Neuron, Polyunsaturated fatty acid",
author = "Mitsuhiro Nakato and Michinori Matsuo and Nozomu Kono and Makoto Arita and Hiroyuki Arai and Jun Ogawa and Noriyuki Kioka and Kazumitsu Ueda",
year = "2015",
month = "10",
day = "1",
doi = "10.1194/jlr.M058164",
language = "English",
volume = "56",
pages = "1880--1890",
journal = "Journal of Lipid Research",
issn = "0022-2275",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "10",

}

TY - JOUR

T1 - Neurite outgrowth stimulation by n-3 and n-6 PUFAs of phospholipids in apoE-containing lipoproteins secreted from glial cells

AU - Nakato, Mitsuhiro

AU - Matsuo, Michinori

AU - Kono, Nozomu

AU - Arita, Makoto

AU - Arai, Hiroyuki

AU - Ogawa, Jun

AU - Kioka, Noriyuki

AU - Ueda, Kazumitsu

PY - 2015/10/1

Y1 - 2015/10/1

N2 - PUFAs, which account for 25-30% of the total fatty acids in the human brain, are important for normal brain development and cognitive function. However, it remains unclear how PUFAs are delivered to neurons and exert their effects. In this study, we demonstrated that n-3 and n-6 PUFAs added to the medium are incorporated into membrane phospholipids of primary glial cells from rat cortices, and then secreted as the fatty acid moiety of phospholipids in apoE-containing lipoproteins (LpEs). Tandem mass spectrometry analysis further showed that LpEs secreted from glial cells contain a variety of metabolites of PUFAs produced in glial cells by elongation and unsaturation. LpEs are absorbed by endocytosis into neurons via LDL receptorrelated protein 1. LpE-containing n-3 and n-6 PUFAs exhibit a strong effect on neurite outgrowth of hippocampal neurons by increasing the number of branches. This study sheds light on the novel role of LpEs in the central nervous system and also a novel pathway in which PUFAs act on neurons.

AB - PUFAs, which account for 25-30% of the total fatty acids in the human brain, are important for normal brain development and cognitive function. However, it remains unclear how PUFAs are delivered to neurons and exert their effects. In this study, we demonstrated that n-3 and n-6 PUFAs added to the medium are incorporated into membrane phospholipids of primary glial cells from rat cortices, and then secreted as the fatty acid moiety of phospholipids in apoE-containing lipoproteins (LpEs). Tandem mass spectrometry analysis further showed that LpEs secreted from glial cells contain a variety of metabolites of PUFAs produced in glial cells by elongation and unsaturation. LpEs are absorbed by endocytosis into neurons via LDL receptorrelated protein 1. LpE-containing n-3 and n-6 PUFAs exhibit a strong effect on neurite outgrowth of hippocampal neurons by increasing the number of branches. This study sheds light on the novel role of LpEs in the central nervous system and also a novel pathway in which PUFAs act on neurons.

KW - Apolipoprotein E

KW - Apolipoprotein E-containing lipoprotein

KW - Low density lipoprotein receptor-related protein 1

KW - Neuron

KW - Polyunsaturated fatty acid

UR - http://www.scopus.com/inward/record.url?scp=84946559582&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84946559582&partnerID=8YFLogxK

U2 - 10.1194/jlr.M058164

DO - 10.1194/jlr.M058164

M3 - Article

C2 - 26239183

AN - SCOPUS:84946559582

VL - 56

SP - 1880

EP - 1890

JO - Journal of Lipid Research

JF - Journal of Lipid Research

SN - 0022-2275

IS - 10

ER -