Neurofibromatosis 2 gene has novel alternative splicings which controls intracellular protein binding

Toru Nishi, Hideo Takeshima, Kazuya Hamada, Kimio Yoshizato, Hisashi Koga, Kyoichi Sato, Keizo Yamamoto, Isao Kitamura, Masato Kochi, Jun Ichi Kuratsu, Hideyuki Saya, Yukitaka Ushio

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Three novel isoforms of the neurofibromatosis 2 (NF2) gene transcripts generated from alternative splicing were identified from normal human brain, schwannoma and glioma tissues. The 3 novel transcripts lack exon 2, exons 2 and 3, exons 2-4, respectively. Recombinant isoform proteins encoded by those new transcripts have lost the previously reported ability to bind 35S-methionine labeled cellular proteins. Two of seven glioblastoma tissues expressed significantly high levels of the shorter transcripts whereas low grade astrocytomas expressed levels similar to those found in normal brain, suggesting that genomic mutation or aberrant alternative splicing of the NF2 gene may contribute to the progression of malignant gliomas.

Original languageEnglish
Pages (from-to)1025-1029
Number of pages5
JournalInternational Journal of Oncology
Volume10
Issue number5
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

Neurofibromatosis 2 Genes
Alternative Splicing
Protein Binding
Exons
Glioma
Protein Isoforms
Neurilemmoma
Astrocytoma
Brain
Glioblastoma
Recombinant Proteins
Methionine
Mutation
Proteins

Keywords

  • alternative splicing
  • binding protein
  • exon skipping
  • glioblastoma
  • NF2 gene

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Nishi, T., Takeshima, H., Hamada, K., Yoshizato, K., Koga, H., Sato, K., ... Ushio, Y. (1997). Neurofibromatosis 2 gene has novel alternative splicings which controls intracellular protein binding. International Journal of Oncology, 10(5), 1025-1029.

Neurofibromatosis 2 gene has novel alternative splicings which controls intracellular protein binding. / Nishi, Toru; Takeshima, Hideo; Hamada, Kazuya; Yoshizato, Kimio; Koga, Hisashi; Sato, Kyoichi; Yamamoto, Keizo; Kitamura, Isao; Kochi, Masato; Kuratsu, Jun Ichi; Saya, Hideyuki; Ushio, Yukitaka.

In: International Journal of Oncology, Vol. 10, No. 5, 1997, p. 1025-1029.

Research output: Contribution to journalArticle

Nishi, T, Takeshima, H, Hamada, K, Yoshizato, K, Koga, H, Sato, K, Yamamoto, K, Kitamura, I, Kochi, M, Kuratsu, JI, Saya, H & Ushio, Y 1997, 'Neurofibromatosis 2 gene has novel alternative splicings which controls intracellular protein binding', International Journal of Oncology, vol. 10, no. 5, pp. 1025-1029.
Nishi T, Takeshima H, Hamada K, Yoshizato K, Koga H, Sato K et al. Neurofibromatosis 2 gene has novel alternative splicings which controls intracellular protein binding. International Journal of Oncology. 1997;10(5):1025-1029.
Nishi, Toru ; Takeshima, Hideo ; Hamada, Kazuya ; Yoshizato, Kimio ; Koga, Hisashi ; Sato, Kyoichi ; Yamamoto, Keizo ; Kitamura, Isao ; Kochi, Masato ; Kuratsu, Jun Ichi ; Saya, Hideyuki ; Ushio, Yukitaka. / Neurofibromatosis 2 gene has novel alternative splicings which controls intracellular protein binding. In: International Journal of Oncology. 1997 ; Vol. 10, No. 5. pp. 1025-1029.
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