Neurofibromatosis 2 gene has novel alternative splicings which controls intracellular protein binding

Toru Nishi, Hideo Takeshima, Kazuya Hamada, Kimio Yoshizato, Hisashi Koga, Kyoichi Sato, Keizo Yamamoto, Isao Kitamura, Masato Kochi, Jun Ichi Kuratsu, Hideyuki Saya, Yukitaka Ushio

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Three novel isoforms of the neurofibromatosis 2 (NF2) gene transcripts generated from alternative splicing were identified from normal human brain, schwannoma and glioma tissues. The 3 novel transcripts lack exon 2, exons 2 and 3, exons 2-4, respectively. Recombinant isoform proteins encoded by those new transcripts have lost the previously reported ability to bind 35S-methionine labeled cellular proteins. Two of seven glioblastoma tissues expressed significantly high levels of the shorter transcripts whereas low grade astrocytomas expressed levels similar to those found in normal brain, suggesting that genomic mutation or aberrant alternative splicing of the NF2 gene may contribute to the progression of malignant gliomas.

Original languageEnglish
Pages (from-to)1025-1029
Number of pages5
JournalInternational journal of oncology
Volume10
Issue number5
DOIs
Publication statusPublished - 1997 Jan 1
Externally publishedYes

Keywords

  • NF2 gene
  • alternative splicing
  • binding protein
  • exon skipping
  • glioblastoma

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Nishi, T., Takeshima, H., Hamada, K., Yoshizato, K., Koga, H., Sato, K., Yamamoto, K., Kitamura, I., Kochi, M., Kuratsu, J. I., Saya, H., & Ushio, Y. (1997). Neurofibromatosis 2 gene has novel alternative splicings which controls intracellular protein binding. International journal of oncology, 10(5), 1025-1029. https://doi.org/10.3892/ijo.10.5.1025