Neuronal cell differentiation of human neuroblastoma cells by retinoic acid plus herbimycin A

P. N. Preis, Hideyuki Saya, L. Nadasdi, G. Hochlaus, V. Levin, W. Sadee

Research output: Contribution to journalArticle

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Abstract

We investigated the effect of retinoic acid (RA) and herbimycin A (herb-A) on cell growth, cell differentiation, and colony formation of human neuroblastoma cell lines. The neuroblastoma line SK-N-SH expressed both neuroblast and nonneuronal phenotypes, whereas its subclone SH-SY5Y and the Kelly cell line were predominantly neuroblastic. Both herb-A and RA, given alone, moderately reduced cell growth and colony formation of the neuroblastic cell lines. Growth curve analyses with SK-N-SH suggested that herb-A greatly reduced the number of initially growing cells, whereas RA slightly enhanced initial cell growth. Morphological changes were determined with the use of rhodamine-phalloidin staining of actin. Retinoic acid caused an increase in the fraction of neuroblast cell in SK-N-SH, and conversely of nonneuronal cells in SH-SY5Y and Kelly cell lines. Both drugs also caused partial differentiation towards a neuronal phenotype, and herb-A induced selective lysis of nonneuronal cells of SK-N-SH. Because of their discrepant effects, RA (10 μM) and herb-A (236 nM) were tested in combination at a concentration that had only moderate effects when given alone. The combination further reduced cell growth and colony formation and dramatically enhanced differentiation towards a neuronal morphology. The Kelly cell line with amplified N-myc genome, which correlates with clinical progression of neuroblastoma, was also sensitive to RA plus herb-A. These results recommend the combination of RA and herb-A for differentiation therapy of neuroblastoma.

Original languageEnglish
Pages (from-to)6530-6534
Number of pages5
JournalCancer Research
Volume48
Issue number22
Publication statusPublished - 1988
Externally publishedYes

Fingerprint

Tretinoin
Neuroblastoma
Cell Differentiation
Cell Line
Growth
Phenotype
herbimycin
Actins
Genome
Staining and Labeling
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Preis, P. N., Saya, H., Nadasdi, L., Hochlaus, G., Levin, V., & Sadee, W. (1988). Neuronal cell differentiation of human neuroblastoma cells by retinoic acid plus herbimycin A. Cancer Research, 48(22), 6530-6534.

Neuronal cell differentiation of human neuroblastoma cells by retinoic acid plus herbimycin A. / Preis, P. N.; Saya, Hideyuki; Nadasdi, L.; Hochlaus, G.; Levin, V.; Sadee, W.

In: Cancer Research, Vol. 48, No. 22, 1988, p. 6530-6534.

Research output: Contribution to journalArticle

Preis, PN, Saya, H, Nadasdi, L, Hochlaus, G, Levin, V & Sadee, W 1988, 'Neuronal cell differentiation of human neuroblastoma cells by retinoic acid plus herbimycin A', Cancer Research, vol. 48, no. 22, pp. 6530-6534.
Preis, P. N. ; Saya, Hideyuki ; Nadasdi, L. ; Hochlaus, G. ; Levin, V. ; Sadee, W. / Neuronal cell differentiation of human neuroblastoma cells by retinoic acid plus herbimycin A. In: Cancer Research. 1988 ; Vol. 48, No. 22. pp. 6530-6534.
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