Neuronal re-juvenilization in the nucleus ambiguus after vagal nerve injury

Takuji Takaoka, Akihiro Shiotani, Koichiro Saito, Masayuki Tomifuji, Yuko Mori, Takekatsu Fujimine, Hideyuki Okano, Kaoru Ogawa

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Nestin is an intermediate filament expressed in immature cells in the CNS including neural stem/progenitor cells, reactive astrocytes and immature neurons in lesser amounts after injury. Nestin expression in the nucleus ambiguus following vagal nerve injury was studied using nestin-EGFP transgenic rats. We confirmed that EGFP immunoreactivity was evident at 6 h to 8 days in ipsilateral nucleus ambiguus after nerve transection. Properties of these cells were examined immunohistochemically. These EGFP-immunoreactive cells were immunoreactive for Tuj1 and Hu, and exhibited ChAT activity. However, no immunoreactivity for GFAP or CNPase was observed. In normal development, the level of KCC2 expression is known to increase with maturation of neurons. In our study, decreased KCC2 expression was observed in nestin-EGFP-positive cells within the nucleus ambiguus on the lesioned side compared with the contralateral side. These EGFP-immunoreactive cells were immunonegative for BrdU. This is the first study to demonstrate the expression of a neural stem/progenitor cell-enriched marker, Nestin, in the nucleus ambiguus after vagal nerve injury. The present findings suggest that mature motoneurons are an origin of these Nestin-positive cells, which are induced after injury. Mature neurons in the nucleus ambiguus may thus have the potential to juvenilize after vagal/recurrent laryngeal nerve injury.

Original languageEnglish
Pages (from-to)353-359
Number of pages7
JournalNeuroscience Research
Volume65
Issue number4
DOIs
Publication statusPublished - 2009 Dec

Fingerprint

Nestin
Medulla Oblongata
Wounds and Injuries
Neural Stem Cells
Neurons
2',3'-Cyclic-Nucleotide Phosphodiesterases
Stem Cells
Recurrent Laryngeal Nerve Injuries
Transgenic Rats
Intermediate Filaments
Motor Neurons
Bromodeoxyuridine
Cell Nucleus
Astrocytes

Keywords

  • Motoneurons
  • Nestin
  • Neural
  • Nucleus ambiguus
  • Peripheral nervous system
  • Progenitors
  • Vagus nerve

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Neuronal re-juvenilization in the nucleus ambiguus after vagal nerve injury. / Takaoka, Takuji; Shiotani, Akihiro; Saito, Koichiro; Tomifuji, Masayuki; Mori, Yuko; Fujimine, Takekatsu; Okano, Hideyuki; Ogawa, Kaoru.

In: Neuroscience Research, Vol. 65, No. 4, 12.2009, p. 353-359.

Research output: Contribution to journalArticle

Takaoka, T, Shiotani, A, Saito, K, Tomifuji, M, Mori, Y, Fujimine, T, Okano, H & Ogawa, K 2009, 'Neuronal re-juvenilization in the nucleus ambiguus after vagal nerve injury', Neuroscience Research, vol. 65, no. 4, pp. 353-359. https://doi.org/10.1016/j.neures.2009.08.012
Takaoka T, Shiotani A, Saito K, Tomifuji M, Mori Y, Fujimine T et al. Neuronal re-juvenilization in the nucleus ambiguus after vagal nerve injury. Neuroscience Research. 2009 Dec;65(4):353-359. https://doi.org/10.1016/j.neures.2009.08.012
Takaoka, Takuji ; Shiotani, Akihiro ; Saito, Koichiro ; Tomifuji, Masayuki ; Mori, Yuko ; Fujimine, Takekatsu ; Okano, Hideyuki ; Ogawa, Kaoru. / Neuronal re-juvenilization in the nucleus ambiguus after vagal nerve injury. In: Neuroscience Research. 2009 ; Vol. 65, No. 4. pp. 353-359.
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