Neutrophil elastase cleaves the murine hemidesmosomal protein BP180/type XVII collagen and generates degradation products that modulate experimental bullous pemphigoid

Lan Lin, Tomoko Betsuyaku, Lisa Heimbach, Ning Li, David Rubenstein, Steven D. Shapiro, Lijia An, George J. Giudice, Luis A. Diaz, Robert M. Senior, Zhi Liu

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Bullous pemphigoid (BP) is an autoimmune subepidermal blistering disease associated with autoantibodies against the hemidesmosomal proteins BP180 and BP230. In the IgG passive transfer model of BP, blister formation is triggered by anti-BP180 IgG and depends on complement activation, mast cell degranulation, and neutrophil recruitment. Mice lacking neutrophil elastase (NE) do not develop experimental BP. Here, we demonstrated that NE degrades recombinant mouse BP180 within the immunodominant extracellular domain at amino acid positions 506 and 561, generating peptide p561 and peptide p506. Peptide p561 is chemotactic for neutrophils both in vitro and in vivo. Local injection of NE into B6 mice recruits neutrophils to the skin, and neutrophil infiltration is completely blocked by co-injection with the NE inhibitor α1-proteinase inhibitor. More importantly, NE directly cleaves BP180 in mouse and human skin, as well as the native human BP180 trimer molecule. These results demonstrate that (i) NE directly damages the extracellular matrix and (ii) NE degradation of mouse BP180 generates neutrophil chemotactic peptides that amplify disease severity at the early stage of the disease.

Original languageEnglish
Pages (from-to)38-44
Number of pages7
JournalMatrix Biology
Volume31
Issue number1
DOIs
Publication statusPublished - 2012 Jan
Externally publishedYes

Fingerprint

Bullous Pemphigoid
Leukocyte Elastase
Peptides
Proteins
Neutrophils
Neutrophil Infiltration
Secretory Proteinase Inhibitory Proteins
Cell Degranulation
Immunodominant Epitopes
Skin
Injections
Complement Activation
Blister
Population Groups
Mast Cells
Autoantibodies
Extracellular Matrix
collagen type XVII
Peptide Hydrolases
Immunoglobulin G

Keywords

  • Autoimmune mouse model
  • Basement membrane
  • BP180
  • Bullous pemphigoid
  • Chemotaxis
  • Neutrophil elastase

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Neutrophil elastase cleaves the murine hemidesmosomal protein BP180/type XVII collagen and generates degradation products that modulate experimental bullous pemphigoid. / Lin, Lan; Betsuyaku, Tomoko; Heimbach, Lisa; Li, Ning; Rubenstein, David; Shapiro, Steven D.; An, Lijia; Giudice, George J.; Diaz, Luis A.; Senior, Robert M.; Liu, Zhi.

In: Matrix Biology, Vol. 31, No. 1, 01.2012, p. 38-44.

Research output: Contribution to journalArticle

Lin, L, Betsuyaku, T, Heimbach, L, Li, N, Rubenstein, D, Shapiro, SD, An, L, Giudice, GJ, Diaz, LA, Senior, RM & Liu, Z 2012, 'Neutrophil elastase cleaves the murine hemidesmosomal protein BP180/type XVII collagen and generates degradation products that modulate experimental bullous pemphigoid', Matrix Biology, vol. 31, no. 1, pp. 38-44. https://doi.org/10.1016/j.matbio.2011.09.003
Lin, Lan ; Betsuyaku, Tomoko ; Heimbach, Lisa ; Li, Ning ; Rubenstein, David ; Shapiro, Steven D. ; An, Lijia ; Giudice, George J. ; Diaz, Luis A. ; Senior, Robert M. ; Liu, Zhi. / Neutrophil elastase cleaves the murine hemidesmosomal protein BP180/type XVII collagen and generates degradation products that modulate experimental bullous pemphigoid. In: Matrix Biology. 2012 ; Vol. 31, No. 1. pp. 38-44.
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abstract = "Bullous pemphigoid (BP) is an autoimmune subepidermal blistering disease associated with autoantibodies against the hemidesmosomal proteins BP180 and BP230. In the IgG passive transfer model of BP, blister formation is triggered by anti-BP180 IgG and depends on complement activation, mast cell degranulation, and neutrophil recruitment. Mice lacking neutrophil elastase (NE) do not develop experimental BP. Here, we demonstrated that NE degrades recombinant mouse BP180 within the immunodominant extracellular domain at amino acid positions 506 and 561, generating peptide p561 and peptide p506. Peptide p561 is chemotactic for neutrophils both in vitro and in vivo. Local injection of NE into B6 mice recruits neutrophils to the skin, and neutrophil infiltration is completely blocked by co-injection with the NE inhibitor α1-proteinase inhibitor. More importantly, NE directly cleaves BP180 in mouse and human skin, as well as the native human BP180 trimer molecule. These results demonstrate that (i) NE directly damages the extracellular matrix and (ii) NE degradation of mouse BP180 generates neutrophil chemotactic peptides that amplify disease severity at the early stage of the disease.",
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