Neutrophil emigration in the lungs, peritoneum, and skin does not require gelatinase B

Tomoko Betsuyaku, J. Michael Shipley, Zhi Liu, Robert M. Senior

Research output: Contribution to journalArticle

125 Citations (Scopus)

Abstract

Polymorphonuclear leukocytes (PMN) release gelatinase B in response to variable stimuli. Gelatinase B degrades basement membrane components in vitro, and inhibition of matrix metalloproteinase activity blunts PMN migration through a prototype basement membrane (Matrigel) and amnionic membranes. Accordingly, it has been speculated that gelatinase B is necessary for PMN emigration. To test this hypothesis we induced acute inflammation in the lungs, peritoneum, and skin in mice with a null mutation of the gelatinase B gene (gelatinase B-/-) and littermate controls (gelatinase B+/+). At 3, 6, 12, and 24 h after intratracheal instillation of LPS, the emigration of PMN in the lung, as determined by PMN in bronchoalveolar lavage fluid, was similar in gelatinase B-/-and gelatinase B+/+ mice. The number of PMN in the peritoneal cavity 4 h after thioglycollate-induced peritonitis was also comparable in gelatinase B-/-and gelatinase B+/+ mice. At 4 h after an intradermal injection of interleukin-8, numerous PMN were present extravascularly in the dermis in both gelatinase B-/-and gelatinase B+/+ mice and the myelo-peroxidase activities of the skin at the injection sites were indistinguishable between the two types of mice. PMN from gelatinase B-/-mice migrated through Matrigel in response to zymosan-activated serum with the same efficiency as did PMN from gelatinase B+/+ mice. In vitro, gelatinase B-/-PMN killed Staphylococcus aureus and Klebsiella pneumoniae as effectively as did PMN from gelatinase B+/+ mice. These findings indicate that gelatinase B is not required for PMN emigration, and suggest that the antibacterial function of PMN is preserved despite gelatinase, B deficiency.

Original languageEnglish
Pages (from-to)1303-1309
Number of pages7
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume20
Issue number6
Publication statusPublished - 1999
Externally publishedYes

Fingerprint

Peritoneum
Emigration and Immigration
Matrix Metalloproteinase 9
Skin
Neutrophils
Lung
Genes
Basement Membrane
Staphylococcal Pneumonia
Thioglycolates
Intradermal Injections
Zymosan
Peritoneal Cavity
Bronchoalveolar Lavage Fluid
Klebsiella pneumoniae
Dermis
Peritonitis
Matrix Metalloproteinases
Interleukin-8

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Pulmonary and Respiratory Medicine

Cite this

Neutrophil emigration in the lungs, peritoneum, and skin does not require gelatinase B. / Betsuyaku, Tomoko; Shipley, J. Michael; Liu, Zhi; Senior, Robert M.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 20, No. 6, 1999, p. 1303-1309.

Research output: Contribution to journalArticle

Betsuyaku, Tomoko ; Shipley, J. Michael ; Liu, Zhi ; Senior, Robert M. / Neutrophil emigration in the lungs, peritoneum, and skin does not require gelatinase B. In: American Journal of Respiratory Cell and Molecular Biology. 1999 ; Vol. 20, No. 6. pp. 1303-1309.
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abstract = "Polymorphonuclear leukocytes (PMN) release gelatinase B in response to variable stimuli. Gelatinase B degrades basement membrane components in vitro, and inhibition of matrix metalloproteinase activity blunts PMN migration through a prototype basement membrane (Matrigel) and amnionic membranes. Accordingly, it has been speculated that gelatinase B is necessary for PMN emigration. To test this hypothesis we induced acute inflammation in the lungs, peritoneum, and skin in mice with a null mutation of the gelatinase B gene (gelatinase B-/-) and littermate controls (gelatinase B+/+). At 3, 6, 12, and 24 h after intratracheal instillation of LPS, the emigration of PMN in the lung, as determined by PMN in bronchoalveolar lavage fluid, was similar in gelatinase B-/-and gelatinase B+/+ mice. The number of PMN in the peritoneal cavity 4 h after thioglycollate-induced peritonitis was also comparable in gelatinase B-/-and gelatinase B+/+ mice. At 4 h after an intradermal injection of interleukin-8, numerous PMN were present extravascularly in the dermis in both gelatinase B-/-and gelatinase B+/+ mice and the myelo-peroxidase activities of the skin at the injection sites were indistinguishable between the two types of mice. PMN from gelatinase B-/-mice migrated through Matrigel in response to zymosan-activated serum with the same efficiency as did PMN from gelatinase B+/+ mice. In vitro, gelatinase B-/-PMN killed Staphylococcus aureus and Klebsiella pneumoniae as effectively as did PMN from gelatinase B+/+ mice. These findings indicate that gelatinase B is not required for PMN emigration, and suggest that the antibacterial function of PMN is preserved despite gelatinase, B deficiency.",
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