Neutrophils activated by granulocyte colony-stimulating factor suppress tumor necrosis factor-alpha release from monocytes stimulated by endotoxin.

T. Terashima, Kenzo Soejima, Y. Waki, H. Nakamura, Seitaro Fujishima, Y. Suzuki, A. Ishizaka, M. Kanazawa

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Abstract

We investigated the in vitro effects of granulocyte colony-stimulating factor (G-CSF) on tumor necrosis factor-alpha (TNF-alpha) release from monocytes. Peripheral blood monocytes and neutrophils were obtained from healthy donors (n = 8). Neutrophils alone, neutrophils plus monocytes, and monocytes alone were incubated with and without G-CSF (10 ng/ml) and were studied for TNF-alpha release; monocytes subsequently were stimulated by endotoxin (lipopolysaccharide [LPS] at 10 and 1,000 ng/ml). Neutrophils alone did not produce TNF-alpha after LPS stimulation irrespective of G-CSF treatment. TNF-alpha release from monocytes was suppressed significantly by pretreatment with G-CSF in the presence of neutrophils (P < 0.01). Suppression of TNF-alpha release after LPS was not observed when monocytes were preincubated with G-CSF in the absence of neutrophils. TNF-alpha release from monocytes stimulated by LPS was not inhibited when monocytes were incubated with the supernatant from G-CSF-activated neutrophils. Pretreatment with G-CSF inhibited intracellular TNF-alpha production, as measured by flow cytometry, of monocytes stimulated by LPS (P < 0.05). These data suggest that neutrophils activated by G-CSF directly suppress TNF-alpha release from monocytes stimulated by LPS.

Original languageEnglish
Pages (from-to)69-73
Number of pages5
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume13
Issue number1
Publication statusPublished - 1995 Jul

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Granulocyte Colony-Stimulating Factor
Endotoxins
Monocytes
Neutrophils
Tumor Necrosis Factor-alpha
Lipopolysaccharides
Flow cytometry
Blood
Flow Cytometry

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Pulmonary and Respiratory Medicine

Cite this

Neutrophils activated by granulocyte colony-stimulating factor suppress tumor necrosis factor-alpha release from monocytes stimulated by endotoxin. / Terashima, T.; Soejima, Kenzo; Waki, Y.; Nakamura, H.; Fujishima, Seitaro; Suzuki, Y.; Ishizaka, A.; Kanazawa, M.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 13, No. 1, 07.1995, p. 69-73.

Research output: Contribution to journalArticle

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