New approach for M-Cell-specific molecules screening by comprehensive transcriptome analysis

Gaku Nakato, Shinji Fukuda, Koji Hase, Ryo Goitsuka, Max D. Cooper, Hiroshi Ohno

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

A minor population of M cells within the follicle-associated epithelium (FAE) of intestinal Peyer's patches (PPs) serves as a major portal for entry of exogenous antigens. Characterization of the mammalian M cells, including identification of M-cell surface molecules used for bacterial uptake, has been hampered by their relative rarity. In contrast, M cells constitute virtually all of the FAE cells in the avian bursa of Fabricius. We therefore performed comparative gene expression profiling of chicken and murine FAE to identify commonly expressed genes by M cells in both species. The comprehensive transcriptome analysis revealed that 28 genes were commonly up-regulated in FAE from both species. In situ hybridization revealed that annexin A10 (Anxa10) mRNA was scattered in FAE, and co-localized with Ulex europaeus agglutinin-1 binding to M cells. Whole-mount immunostaining also revealed that cellular prion protein (PrPC) was expressed on the luminal side of the apical plasma membrane of M cells, and co-localized with grycoprotein 2 that recognizes only M cells in murine PP. Our findings identify new M-cell-specific molecules through using comprehensive transcriptome analysis. These conserved molecules in M cells of mice and chickens may play essential roles in M-cell function and/or differentiation.

Original languageEnglish
Pages (from-to)227-235
Number of pages9
JournalDNA Research
Volume16
Issue number4
DOIs
Publication statusPublished - 2009 Aug 1
Externally publishedYes

Keywords

  • Annexin A10
  • Cellular prion protein
  • M cell
  • The bursa of Fabricius

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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