New approaches to in vitro models of blood-brain barrier drug transport

Tetsuya Terasaki, Sumio Ohtsuki, Satoko Hori, Hitomi Takanaga, Emi Nakashima, Ken Ichi Hosoya

Research output: Contribution to journalArticle

135 Citations (Scopus)

Abstract

The pharmaceutical industry has been searching for an in vitro blood-brain barrier (BBB) model that preserves in vivo transporter functions in CNS drug discovery and development. The application of conditionally immortalized cell lines derived from transgenic animals harboring temperature-sensitive SV40 large T-antigen gene, is a rational and promising approach to such a workable in vitro BBB model. The established brain capillary endothelial cell lines retain the in vivo transport rate of several compounds and various forms of gene expression. Furthermore, this new approach has enabled the development of stable and reproducible co-culture models with a pericyte cell line and/or an astrocyte cell line.

Original languageEnglish
Pages (from-to)944-954
Number of pages11
JournalDrug Discovery Today
Volume8
Issue number20
DOIs
Publication statusPublished - 2003 Oct 15

Fingerprint

Blood-Brain Barrier
Cell Line
Pharmaceutical Preparations
Polyomavirus Transforming Antigens
Pericytes
Genetically Modified Animals
Viral Tumor Antigens
Drug Industry
Drug Discovery
Coculture Techniques
Astrocytes
Endothelial Cells
Gene Expression
Temperature
In Vitro Techniques
Brain
Genes

ASJC Scopus subject areas

  • Drug Discovery
  • Pharmacology

Cite this

Terasaki, T., Ohtsuki, S., Hori, S., Takanaga, H., Nakashima, E., & Hosoya, K. I. (2003). New approaches to in vitro models of blood-brain barrier drug transport. Drug Discovery Today, 8(20), 944-954. https://doi.org/10.1016/S1359-6446(03)02858-7

New approaches to in vitro models of blood-brain barrier drug transport. / Terasaki, Tetsuya; Ohtsuki, Sumio; Hori, Satoko; Takanaga, Hitomi; Nakashima, Emi; Hosoya, Ken Ichi.

In: Drug Discovery Today, Vol. 8, No. 20, 15.10.2003, p. 944-954.

Research output: Contribution to journalArticle

Terasaki, T, Ohtsuki, S, Hori, S, Takanaga, H, Nakashima, E & Hosoya, KI 2003, 'New approaches to in vitro models of blood-brain barrier drug transport', Drug Discovery Today, vol. 8, no. 20, pp. 944-954. https://doi.org/10.1016/S1359-6446(03)02858-7
Terasaki, Tetsuya ; Ohtsuki, Sumio ; Hori, Satoko ; Takanaga, Hitomi ; Nakashima, Emi ; Hosoya, Ken Ichi. / New approaches to in vitro models of blood-brain barrier drug transport. In: Drug Discovery Today. 2003 ; Vol. 8, No. 20. pp. 944-954.
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