New Cdc25B tyrosine phosphatase inhibitors, nocardiones A and B, produced by Nocardia sp. TP-A0248

Taxonomy, fermentation, isolation, structural elucidation and biological properties

Toshio Otani, Yoshikazu Sugimoto, Yoshimi Aoyagi, Yasuhiro Igarashi, Tamotsu Furumai, Noriko Saito, Yuji Yamada, Tetsuji Asao, Toshikazu Oki

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Strain TP-A0248 which produces two new Cdc25B tyrosine phosphatase inhibitors and also possessing antifungal activity, designated nocardiones A (1) and B (2), was considered to belong to the genus Nocardia on the basis of literature comparison of chemotaxonomic properties. The nocardiones were isolated by solvent extraction of fermentation broth of Nocardia sp. TP-A0248 and purified by the conventional column chromatography. Spectroscopic studies led to determination that 1 and 2 belong to a class compound of naphtho[1,2-b]furan-4,5-diones. Compound 1 inhibited the activity of Cdc25B, PTP1B and FAP-1 protein tyrosine phosphatases at a concentration of 10 μM. It also showed moderate in vitro antifungal and cytotoxic activity.

Original languageEnglish
Pages (from-to)337-344
Number of pages8
JournalJournal of Antibiotics
Volume53
Issue number4
Publication statusPublished - 2000 Apr
Externally publishedYes

Fingerprint

cdc25 Phosphatases
Nocardia
Fermentation
Tyrosine
Protein Tyrosine Phosphatases
Chromatography
nocardione A
nocardione B

ASJC Scopus subject areas

  • Pharmacology
  • Molecular Medicine

Cite this

New Cdc25B tyrosine phosphatase inhibitors, nocardiones A and B, produced by Nocardia sp. TP-A0248 : Taxonomy, fermentation, isolation, structural elucidation and biological properties. / Otani, Toshio; Sugimoto, Yoshikazu; Aoyagi, Yoshimi; Igarashi, Yasuhiro; Furumai, Tamotsu; Saito, Noriko; Yamada, Yuji; Asao, Tetsuji; Oki, Toshikazu.

In: Journal of Antibiotics, Vol. 53, No. 4, 04.2000, p. 337-344.

Research output: Contribution to journalArticle

Otani, Toshio ; Sugimoto, Yoshikazu ; Aoyagi, Yoshimi ; Igarashi, Yasuhiro ; Furumai, Tamotsu ; Saito, Noriko ; Yamada, Yuji ; Asao, Tetsuji ; Oki, Toshikazu. / New Cdc25B tyrosine phosphatase inhibitors, nocardiones A and B, produced by Nocardia sp. TP-A0248 : Taxonomy, fermentation, isolation, structural elucidation and biological properties. In: Journal of Antibiotics. 2000 ; Vol. 53, No. 4. pp. 337-344.
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AU - Igarashi, Yasuhiro

AU - Furumai, Tamotsu

AU - Saito, Noriko

AU - Yamada, Yuji

AU - Asao, Tetsuji

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