New micro-glass-tube leukocyte adherence inhibition assay assessing cell adherence of mononuclear cell subpopulations defined by monoclonal antibodies

Toshio Morizane, Itsurou Satoh, Naoki Kumagai, Hidetsugu Saito, Akiko Iino, Yasutaka Inagaki, Tetsu Watanabe, Kanji Tsuchimoto, Masaharu Tsuchiya

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A new micro-glass-tube leukocyte adherence inhibition (LAI) assay which is appropriate for detecting delayed type hypersensitivity in vitro has been developed for human leukocytes. Enumeration of adherent cells is replaced by a cellular radioimmunoassay determining antibody binding of the monoclonal reagents, OKT4, OKT8, and OKM1, to glass-adherent cells, fixed by glutaraldehyde or formaldehyde. An LAI reactivity to purified protein derivative of tuberculin (PPD) was detectable in donors giving a positive PPD skin test with OKT4 reagent, but not with the other two reagents.

Original languageEnglish
Pages (from-to)225-229
Number of pages5
JournalJournal of Immunological Methods
Volume91
Issue number2
DOIs
Publication statusPublished - 1986 Jul 24

Keywords

  • Delayed-type hypersensitivity
  • Leukocyte adherence inhibition
  • Purified protein derivative of tuberculin

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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