New role of δ2-glutamate receptors in AMPA receptor trafficking and cerebellar function

Hirokazu Hirai, Thomas Launey, Sumiko Mikawa, Takashi Torashima, Dai Yanagihara, Tsuyoshi Kasaura, Akihiro Miyamoto, Michisuke Yuzaki

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100 Citations (Scopus)

Abstract

Previous gene knockout studies have shown that the orphan glutamate receptor δ2 (GluRδ2) is critically involved in synaptogenesis between parallel fibers and Purkinje cells during development. However, the precise function of GluRδ2 and whether it is functional in the mature cerebellum remain unclear. To address these issues, we developed an antibody specific for the putative ligand-binding region of GluRδ2, and application of this antibody to cultured Purkinje cells induced AMPA receptor endocytosis, attenuated synaptic transmission and abrogated long-term depression. Moreover, injection of this antibody into the subarachnoidal supracerebellar space of adult mice caused transient cerebellar dysfunction, such as ataxic gait and poor performance in the rotorod test. These results indicate that GluRδ2 is involved in AMPA receptor trafficking and cerebellar function in adult mice.

Original languageEnglish
Pages (from-to)869-876
Number of pages8
JournalNature Neuroscience
Volume6
Issue number8
DOIs
Publication statusPublished - 2003 Aug 1

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ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Hirai, H., Launey, T., Mikawa, S., Torashima, T., Yanagihara, D., Kasaura, T., Miyamoto, A., & Yuzaki, M. (2003). New role of δ2-glutamate receptors in AMPA receptor trafficking and cerebellar function. Nature Neuroscience, 6(8), 869-876. https://doi.org/10.1038/nn1086