New single-stranded DNA virus with a unique genomic structure that infects marine diatom Chaetoceros setoensis

Yuji Tomaru, Kensuke Toyoda, Hidekazu Suzuki, Tamotsu Nagumo, Kei Kimura, Yoshitake Takao

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18 Citations (Scopus)

Abstract

Diatoms are among the most abundant organisms in nature; however, their relationships with single-stranded DNA (ssDNA) viruses have not yet been defined in detail. We report the isolation and characterisation of a virus (CsetDNAV) that lytically infects the bloom-forming diatom Chaetoceros setoensis. The virion is 33ânm in diameter and accumulates in the nucleus of its host. CsetDNAV harbours a covalently closed-circular ssDNA genome comprising 5836 nucleotides and eight different short-complementary fragments (67-145 nucleotides), which have not been reported in other diatom viruses. Phylogenetic analysis based on the putative replicase-related protein showed that CsetDNAV was not included in the monophyly of the recently established genus Bacilladnavirus. This discovery of CsetDNAV, which harbours a genome with a structure that is unique among known viruses that infect diatoms, suggests that other such undiscovered viruses possess diverse genomic architectures.

Original languageEnglish
Article number3337
JournalScientific reports
Volume3
DOIs
Publication statusPublished - 2013 Dec 4

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