New single-stranded DNA virus with a unique genomic structure that infects marine diatom Chaetoceros setoensis

Yuji Tomaru, Kensuke Toyoda, Hidekazu Suzuki, Tamotsu Nagumo, Kei Kimura, Yoshitake Takao

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Diatoms are among the most abundant organisms in nature; however, their relationships with single-stranded DNA (ssDNA) viruses have not yet been defined in detail. We report the isolation and characterisation of a virus (CsetDNAV) that lytically infects the bloom-forming diatom Chaetoceros setoensis. The virion is 33ânm in diameter and accumulates in the nucleus of its host. CsetDNAV harbours a covalently closed-circular ssDNA genome comprising 5836 nucleotides and eight different short-complementary fragments (67-145 nucleotides), which have not been reported in other diatom viruses. Phylogenetic analysis based on the putative replicase-related protein showed that CsetDNAV was not included in the monophyly of the recently established genus Bacilladnavirus. This discovery of CsetDNAV, which harbours a genome with a structure that is unique among known viruses that infect diatoms, suggests that other such undiscovered viruses possess diverse genomic architectures.

Original languageEnglish
Article number3337
JournalScientific Reports
Volume3
DOIs
Publication statusPublished - 2013

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Diatoms
DNA Viruses
Single-Stranded DNA
Viruses
Nucleotides
Genome
Circular DNA
Virion
Proteins

ASJC Scopus subject areas

  • General

Cite this

New single-stranded DNA virus with a unique genomic structure that infects marine diatom Chaetoceros setoensis. / Tomaru, Yuji; Toyoda, Kensuke; Suzuki, Hidekazu; Nagumo, Tamotsu; Kimura, Kei; Takao, Yoshitake.

In: Scientific Reports, Vol. 3, 3337, 2013.

Research output: Contribution to journalArticle

Tomaru, Yuji ; Toyoda, Kensuke ; Suzuki, Hidekazu ; Nagumo, Tamotsu ; Kimura, Kei ; Takao, Yoshitake. / New single-stranded DNA virus with a unique genomic structure that infects marine diatom Chaetoceros setoensis. In: Scientific Reports. 2013 ; Vol. 3.
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