Newly developed rat brain pericyte cell line, TR-PCT1, responds to transforming growth factor-β1 and β-glycerophosphate

Tomoko Asashima, Hisashi Iizasa, Tetsuya Terasaki, Ken Ichi Hosoya, Kazuhiro Tetsuka, Masatsugu Ueda, Masuo Obinata, Emi Nakashima

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Brain pericytes form an incomplete envelope around endothelial cells and within the microvascular basement membrane of capillaries and postcapillary venules. Recently, it has been reported that brain pericytes exhibit pluripotency, regulation of endothelial cell activity, and macrophage activity. However, many molecular and cellular aspects of brain pericytes remain unclear. In this study, we have tried to establish a conditionally immortalized brain pericyte cell line (TR-PCT) derived from the brain capillary of a transgenic rat harboring a temperature-sensitive simian virus 40 T antigen gene. One of the clones was named TR-PCT1, and we established 6 clones of pericyte-like cells from a 16 week-old tsA58 transgenic rat. For comparison, primary pericytes from a Wistar rat were also studied. The expression of platelet-derived growth factor receptor β, angiopoietin-1, osteopontin, and intercellular adhesion molecule-1 in TR-PCT1 was determined by reverse transcription-polymerase chain reaction. Transforming growth factor-β1 enhanced α-smooth muscle actin expression in TR-PCT1, but this expression was reduced by subsequent treatment with basic fibroblast growth factor. When TR-PCT1 was seeded on type I collagen plates and treated with β-glycerophosphate, nodules developed in the cells and these nodules reacted positively to von Kossa stain used as a marker of calcification. We believe that TR-PCT1 will help us gain a better understanding of the physiological and/or pathophysiological role of pericytes.

Original languageEnglish
Pages (from-to)145-152
Number of pages8
JournalEuropean Journal of Cell Biology
Volume81
Issue number3
Publication statusPublished - 2002

Fingerprint

Glycerophosphates
Pericytes
Transforming Growth Factors
Cell Line
Brain
Transgenic Rats
Endothelial Cells
Clone Cells
Angiopoietin-1
Platelet-Derived Growth Factor Receptors
Osteopontin
Simian virus 40
Venules
Viral Tumor Antigens
Fibroblast Growth Factor 2
Intercellular Adhesion Molecule-1
Collagen Type I
Basement Membrane
Reverse Transcription
Smooth Muscle

Keywords

  • Brain pericyte
  • Calcification
  • Platelet-derived growth factor receptor β
  • Temperature-sensitive simian virus T antigen
  • Transforming growth factor-β1

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Asashima, T., Iizasa, H., Terasaki, T., Hosoya, K. I., Tetsuka, K., Ueda, M., ... Nakashima, E. (2002). Newly developed rat brain pericyte cell line, TR-PCT1, responds to transforming growth factor-β1 and β-glycerophosphate. European Journal of Cell Biology, 81(3), 145-152.

Newly developed rat brain pericyte cell line, TR-PCT1, responds to transforming growth factor-β1 and β-glycerophosphate. / Asashima, Tomoko; Iizasa, Hisashi; Terasaki, Tetsuya; Hosoya, Ken Ichi; Tetsuka, Kazuhiro; Ueda, Masatsugu; Obinata, Masuo; Nakashima, Emi.

In: European Journal of Cell Biology, Vol. 81, No. 3, 2002, p. 145-152.

Research output: Contribution to journalArticle

Asashima, T, Iizasa, H, Terasaki, T, Hosoya, KI, Tetsuka, K, Ueda, M, Obinata, M & Nakashima, E 2002, 'Newly developed rat brain pericyte cell line, TR-PCT1, responds to transforming growth factor-β1 and β-glycerophosphate', European Journal of Cell Biology, vol. 81, no. 3, pp. 145-152.
Asashima, Tomoko ; Iizasa, Hisashi ; Terasaki, Tetsuya ; Hosoya, Ken Ichi ; Tetsuka, Kazuhiro ; Ueda, Masatsugu ; Obinata, Masuo ; Nakashima, Emi. / Newly developed rat brain pericyte cell line, TR-PCT1, responds to transforming growth factor-β1 and β-glycerophosphate. In: European Journal of Cell Biology. 2002 ; Vol. 81, No. 3. pp. 145-152.
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