NF-Y is essential for the recruitment of RNA polymerase II and inducible transcription of several CCAAT box-containing genes

Yasuaki Kabe, Joe Yamada, Hitoshi Uga, Yuki Yamaguchi, Tadashi Wada, Hiroshi Handa

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Osteoclast differentiation factor (ODF)/receptor activator of NF-κB ligand is essential for inducing the differentiation of mature osteoclasts. We find that nuclear factor Y (NF-Y) binds to the CCAAT box on the ODF promoter and regulates its basal transcriptional activity. The CCAAT box on the ODF gene is required for its transcriptional induction by vitamin D3, suggesting that NF-Y coregulates this promoter along with VDR. Chromatin immunoprecipitation analysis reveals that NF-Y is required for the recruitment of RNA polymerase II (RNAPII) and TATA box binding protein on the ODF promoter. Stimulation with vitamin D3 facilitates the recruitment of VDR and p300 onto the ODF promoter, resulting in acetylation of histone H4 in an NF-Y-independent manner. ODF gene induction by parathyroid hormone or prostaglandin E is also dependent on NF-Y. Furthermore, NF-Y is essential for the recruitment of RNAPII onto other CCAAT box-containing promoters, such as those of osteopontin, CYP24, and E2F1. These results suggest that NF-Y recruits RNAPII and general transcription factors onto various CCAAT box-containing promoters in response to various inductions to permit strong transcriptional activation independently of histone modifications.

Original languageEnglish
Pages (from-to)512-522
Number of pages11
JournalMolecular and Cellular Biology
Volume25
Issue number1
DOIs
Publication statusPublished - 2005 Jan
Externally publishedYes

Fingerprint

RANK Ligand
RNA Polymerase II
TATA-Box Binding Protein
Genes
Cholecalciferol
Histone Code
General Transcription Factors
Osteopontin
Chromatin Immunoprecipitation
Osteoclasts
Acetylation
Prostaglandins E
Parathyroid Hormone
Histones
Transcriptional Activation
nuclear factor Y
Ligands

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

NF-Y is essential for the recruitment of RNA polymerase II and inducible transcription of several CCAAT box-containing genes. / Kabe, Yasuaki; Yamada, Joe; Uga, Hitoshi; Yamaguchi, Yuki; Wada, Tadashi; Handa, Hiroshi.

In: Molecular and Cellular Biology, Vol. 25, No. 1, 01.2005, p. 512-522.

Research output: Contribution to journalArticle

Kabe, Yasuaki ; Yamada, Joe ; Uga, Hitoshi ; Yamaguchi, Yuki ; Wada, Tadashi ; Handa, Hiroshi. / NF-Y is essential for the recruitment of RNA polymerase II and inducible transcription of several CCAAT box-containing genes. In: Molecular and Cellular Biology. 2005 ; Vol. 25, No. 1. pp. 512-522.
@article{654cf5d9787740cdb3730c3bad134730,
title = "NF-Y is essential for the recruitment of RNA polymerase II and inducible transcription of several CCAAT box-containing genes",
abstract = "Osteoclast differentiation factor (ODF)/receptor activator of NF-κB ligand is essential for inducing the differentiation of mature osteoclasts. We find that nuclear factor Y (NF-Y) binds to the CCAAT box on the ODF promoter and regulates its basal transcriptional activity. The CCAAT box on the ODF gene is required for its transcriptional induction by vitamin D3, suggesting that NF-Y coregulates this promoter along with VDR. Chromatin immunoprecipitation analysis reveals that NF-Y is required for the recruitment of RNA polymerase II (RNAPII) and TATA box binding protein on the ODF promoter. Stimulation with vitamin D3 facilitates the recruitment of VDR and p300 onto the ODF promoter, resulting in acetylation of histone H4 in an NF-Y-independent manner. ODF gene induction by parathyroid hormone or prostaglandin E is also dependent on NF-Y. Furthermore, NF-Y is essential for the recruitment of RNAPII onto other CCAAT box-containing promoters, such as those of osteopontin, CYP24, and E2F1. These results suggest that NF-Y recruits RNAPII and general transcription factors onto various CCAAT box-containing promoters in response to various inductions to permit strong transcriptional activation independently of histone modifications.",
author = "Yasuaki Kabe and Joe Yamada and Hitoshi Uga and Yuki Yamaguchi and Tadashi Wada and Hiroshi Handa",
year = "2005",
month = "1",
doi = "10.1128/MCB.25.1.512-522.2005",
language = "English",
volume = "25",
pages = "512--522",
journal = "Molecular and Cellular Biology",
issn = "0270-7306",
publisher = "American Society for Microbiology",
number = "1",

}

TY - JOUR

T1 - NF-Y is essential for the recruitment of RNA polymerase II and inducible transcription of several CCAAT box-containing genes

AU - Kabe, Yasuaki

AU - Yamada, Joe

AU - Uga, Hitoshi

AU - Yamaguchi, Yuki

AU - Wada, Tadashi

AU - Handa, Hiroshi

PY - 2005/1

Y1 - 2005/1

N2 - Osteoclast differentiation factor (ODF)/receptor activator of NF-κB ligand is essential for inducing the differentiation of mature osteoclasts. We find that nuclear factor Y (NF-Y) binds to the CCAAT box on the ODF promoter and regulates its basal transcriptional activity. The CCAAT box on the ODF gene is required for its transcriptional induction by vitamin D3, suggesting that NF-Y coregulates this promoter along with VDR. Chromatin immunoprecipitation analysis reveals that NF-Y is required for the recruitment of RNA polymerase II (RNAPII) and TATA box binding protein on the ODF promoter. Stimulation with vitamin D3 facilitates the recruitment of VDR and p300 onto the ODF promoter, resulting in acetylation of histone H4 in an NF-Y-independent manner. ODF gene induction by parathyroid hormone or prostaglandin E is also dependent on NF-Y. Furthermore, NF-Y is essential for the recruitment of RNAPII onto other CCAAT box-containing promoters, such as those of osteopontin, CYP24, and E2F1. These results suggest that NF-Y recruits RNAPII and general transcription factors onto various CCAAT box-containing promoters in response to various inductions to permit strong transcriptional activation independently of histone modifications.

AB - Osteoclast differentiation factor (ODF)/receptor activator of NF-κB ligand is essential for inducing the differentiation of mature osteoclasts. We find that nuclear factor Y (NF-Y) binds to the CCAAT box on the ODF promoter and regulates its basal transcriptional activity. The CCAAT box on the ODF gene is required for its transcriptional induction by vitamin D3, suggesting that NF-Y coregulates this promoter along with VDR. Chromatin immunoprecipitation analysis reveals that NF-Y is required for the recruitment of RNA polymerase II (RNAPII) and TATA box binding protein on the ODF promoter. Stimulation with vitamin D3 facilitates the recruitment of VDR and p300 onto the ODF promoter, resulting in acetylation of histone H4 in an NF-Y-independent manner. ODF gene induction by parathyroid hormone or prostaglandin E is also dependent on NF-Y. Furthermore, NF-Y is essential for the recruitment of RNAPII onto other CCAAT box-containing promoters, such as those of osteopontin, CYP24, and E2F1. These results suggest that NF-Y recruits RNAPII and general transcription factors onto various CCAAT box-containing promoters in response to various inductions to permit strong transcriptional activation independently of histone modifications.

UR - http://www.scopus.com/inward/record.url?scp=11144335986&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=11144335986&partnerID=8YFLogxK

U2 - 10.1128/MCB.25.1.512-522.2005

DO - 10.1128/MCB.25.1.512-522.2005

M3 - Article

C2 - 15601870

AN - SCOPUS:11144335986

VL - 25

SP - 512

EP - 522

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

SN - 0270-7306

IS - 1

ER -