Nicardipine and itraconazole inhibited transcellular transport of digoxin

Kohji Takara, Yusuke Tanigawara, Fusao Komada, Kohshi Nishiguchi, Toshiyuki Sakaeda, Katsuhiko Okumura

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The inhibitory effects of nicardipine, nifedipine and itraconazole on P- glycoprotein-mediated transport of [3H]digoxin were examined using LLC-PK1 and LLC-GA5-COL150 cells, a porcine kidney epithelial LLC-PK1 cell line transformed with MDR1 cDNA from man which results in overexpression of P- glycoprotein on the apical membrane. Basal-to-apical transport of [3H]digoxin in LLC-GA5-COL150 cells was higher than in LLC-PK1 cells; apical-to-basal transport was markedly lower in LLC-PK1 cells and even lower in LLC-GA5-COL150 cells. This is consistent with the possibility that [3H]digoxin is transported by P-glycoprotein. Co-administration of nicardipine or itraconazole markedly inhibited the basal-to-apical transport of [3H]digoxin in LLC-GA5-COL150 cells, and apical-to-basal transport also increased. The effect of nifedipine was less marked than that of nicardipine or itraconazole. Intracellular accumulation of [3H]digoxin after apical application in LLC-GA5-COL150 cells was 2.3 times less than in LLC-PK1 cells, and was increased by the addition of nicardipine or itraconazole, consistent with their inhibitory effects on transcellular transport. Following basal application of [3H]digoxin, its intracellular accumulation in LLC-GA5-COL150 cells was, unexpectedly, comparable with that in LLC-PK1 cells, and was hardly affected by the addition of nicardipine or itraconazole. In conclusion, it has been shown that nicardipine and itraconazole inhibited transport of digoxin, which is presumably mediated by P-glycoprotein. This explains their effects observed in clinical use.

Original languageEnglish
Pages (from-to)167-171
Number of pages5
JournalPharmacy and Pharmacology Communications
Volume6
Issue number4
Publication statusPublished - 2000 Apr

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Transcytosis
Nicardipine
Itraconazole
Digoxin
LLC-PK1 Cells
P-Glycoprotein
Nifedipine
Swine
Complementary DNA
Epithelial Cells
Kidney
Cell Line
Membranes

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Pharmacology

Cite this

Takara, K., Tanigawara, Y., Komada, F., Nishiguchi, K., Sakaeda, T., & Okumura, K. (2000). Nicardipine and itraconazole inhibited transcellular transport of digoxin. Pharmacy and Pharmacology Communications, 6(4), 167-171.

Nicardipine and itraconazole inhibited transcellular transport of digoxin. / Takara, Kohji; Tanigawara, Yusuke; Komada, Fusao; Nishiguchi, Kohshi; Sakaeda, Toshiyuki; Okumura, Katsuhiko.

In: Pharmacy and Pharmacology Communications, Vol. 6, No. 4, 04.2000, p. 167-171.

Research output: Contribution to journalArticle

Takara, K, Tanigawara, Y, Komada, F, Nishiguchi, K, Sakaeda, T & Okumura, K 2000, 'Nicardipine and itraconazole inhibited transcellular transport of digoxin', Pharmacy and Pharmacology Communications, vol. 6, no. 4, pp. 167-171.
Takara, Kohji ; Tanigawara, Yusuke ; Komada, Fusao ; Nishiguchi, Kohshi ; Sakaeda, Toshiyuki ; Okumura, Katsuhiko. / Nicardipine and itraconazole inhibited transcellular transport of digoxin. In: Pharmacy and Pharmacology Communications. 2000 ; Vol. 6, No. 4. pp. 167-171.
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