Nicotinamide adenine dinucleotide-dependent retinoic acid formation from retinol in the human gastric mucosa: Inhibition by ethanol, acetaldehyde, and H2 blockers

Hirokazu Yokoyama, Michinaga Matsumoto, Haruko Shiraishi, Michiko Miyagi, Shinzo Kato, Hiromasa Ishii

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Abstract

All-trans retinoic acid formation from all-trans retinol (vitamin A) in the human gastric mucosa was studied. When all-trans retinol and the human gastric mucosa were incubated together, all-trans retinoic acid was formed in the presence of nicotinamide adenine dinucleotide (NAD). When the NAD was not added, hardly any formation was observed. The formation of all-trans retinoic acid tended to be attenuated by 10 mM ethanol. Moreover, it was significantly attenuated in a concentration-dependent manner by ethanol at concentrations of 100 mM and above. Acetaldehyde at concentrations of 50 μM and above also significantly attenuated its formation in a concentration-dependent manner. Some H2 blockers, which include ranitidine hydrochloride and cimetidine, significantly attenuated the formation of all-trans retinoic acid, whereas famotidine failed to suppress it. There is an NAD-dependent pathway by which all-trans retinoic acid is produced from all-trans retinol in the human gastric mucosa. Inhibitors of alcohol dehydrogenase, which include ethanol and some H2 blockers, and of aldehyde dehydrogenase, which include acetaldehyde, inhibit its production.

Original languageEnglish
Pages (from-to)24S-28S
JournalAlcoholism: Clinical and Experimental Research
Volume25
Issue number6 SUPPL.
Publication statusPublished - 2001 Jul 3

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Keywords

  • Histamine Receptor Blocker
  • Retinoids
  • Stomach

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Toxicology
  • Psychiatry and Mental health

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