Nimbolide B and nimbic Acid B, phytotoxic substances in neem leaves with allelopathic activity

Hisashi Kato-Noguchi, Md Abdus Salam, Osamu Ohno, Kiyotake Suenaga

Research output: Contribution to journalArticle

13 Citations (Scopus)


Neem (Azadirachta indica) has been widely used as a traditional medicine and several bioactive compounds have been isolated from this species, but to date no potent allelopathic active substance has been reported. Therefore, we investigated possible allelopathic property and phytotoxic substances with allelopathic activity in neem. An aqueous methanol extract of neem leaves inhibited the growth of roots and shoots of cress, lettuce, alfalfa, timothy, crabgrass, ryegrass, barnyard grass and jungle rice. The extracts were then purified by several chromatographic runs while monitoring the inhibitory activity and two phytotoxic substances were isolated. The chemical structures of the two substances were determined by spectral data to correspond to novel compounds, nimbolide B (1) and nimbic acid B (2). Nimbolide B inhibited the growth of cress and barnyard grass at concentrations greater than μM. Nimbic acid B inhibited the growth of cress and barnyard grass at concentrations greater than μM. These results suggest that nimbolide B and nimbic acid B may contribute to the allelopathic effects caused by neem leaves.

Original languageEnglish
Pages (from-to)6929-6940
Number of pages12
Issue number6
Publication statusPublished - 2014 Jun



  • Allelopathy
  • Azadirachta indica
  • Bioactive compound
  • Growth inhibitor
  • Medicinal plant
  • Phytotoxicity

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry

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