Nitric oxide mediates vasoactive effects of endothelin-3 on rat mesenteric microvascular beds in vivo

I. Kurose, D. Fukumura, S. Miura, E. Sekizuka, H. Nagata, M. Suematsu, M. Tsuchiya

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In order to clarify whether the vasoactive effects of endothelin-3 (ET-3) on microvessels are associated with the endothelium-derived relaxing factor (EDRF) in vivo, the authors examined the effects of L-N(G)-monomethyl arginine (L-NMMA), an analog of L-arginine, on low-dose ET-3 induced hemodynamic changes in the mesenteric microcirculation of male Wistar rats. The intravital observation revealed that ET-3 100 pM induced a remarkable and periodic vasoconstriction in arterioles, and the constriction was sustained for approximately fifteen minutes. No remarkable change was observed in the microvessels after the superfusion of 1 pM ET-3. Superfusion of 1 pM ET-3 with 100 μM L-NMMA elicited the vasoconstriction in arterioles, and the arteriolar diameter recovered to the control level within ten minutes in spite of continuing the superfusion. The vasoconstriction induced by low- dose ET-3 with L-NMMA was suppressed by the additional superfusion of 200 μM L-arginine. The present study suggests that the recovery of arteriolar diameter after the ET-3-induced constriction may be mediated by nitric oxide at least in the early phase.

Original languageEnglish
Pages (from-to)483-490
Number of pages8
JournalAngiology
Volume44
Issue number6
Publication statusPublished - 1993

Fingerprint

Endothelin-3
Nitric Oxide
Arginine
Vasoconstriction
Arterioles
Microvessels
Constriction
Endothelium-Dependent Relaxing Factors
Microcirculation
Wistar Rats
Hemodynamics
Observation

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Kurose, I., Fukumura, D., Miura, S., Sekizuka, E., Nagata, H., Suematsu, M., & Tsuchiya, M. (1993). Nitric oxide mediates vasoactive effects of endothelin-3 on rat mesenteric microvascular beds in vivo. Angiology, 44(6), 483-490.

Nitric oxide mediates vasoactive effects of endothelin-3 on rat mesenteric microvascular beds in vivo. / Kurose, I.; Fukumura, D.; Miura, S.; Sekizuka, E.; Nagata, H.; Suematsu, M.; Tsuchiya, M.

In: Angiology, Vol. 44, No. 6, 1993, p. 483-490.

Research output: Contribution to journalArticle

Kurose, I, Fukumura, D, Miura, S, Sekizuka, E, Nagata, H, Suematsu, M & Tsuchiya, M 1993, 'Nitric oxide mediates vasoactive effects of endothelin-3 on rat mesenteric microvascular beds in vivo', Angiology, vol. 44, no. 6, pp. 483-490.
Kurose I, Fukumura D, Miura S, Sekizuka E, Nagata H, Suematsu M et al. Nitric oxide mediates vasoactive effects of endothelin-3 on rat mesenteric microvascular beds in vivo. Angiology. 1993;44(6):483-490.
Kurose, I. ; Fukumura, D. ; Miura, S. ; Sekizuka, E. ; Nagata, H. ; Suematsu, M. ; Tsuchiya, M. / Nitric oxide mediates vasoactive effects of endothelin-3 on rat mesenteric microvascular beds in vivo. In: Angiology. 1993 ; Vol. 44, No. 6. pp. 483-490.
@article{809f990ca43a44df9b8a206af09165c6,
title = "Nitric oxide mediates vasoactive effects of endothelin-3 on rat mesenteric microvascular beds in vivo",
abstract = "In order to clarify whether the vasoactive effects of endothelin-3 (ET-3) on microvessels are associated with the endothelium-derived relaxing factor (EDRF) in vivo, the authors examined the effects of L-N(G)-monomethyl arginine (L-NMMA), an analog of L-arginine, on low-dose ET-3 induced hemodynamic changes in the mesenteric microcirculation of male Wistar rats. The intravital observation revealed that ET-3 100 pM induced a remarkable and periodic vasoconstriction in arterioles, and the constriction was sustained for approximately fifteen minutes. No remarkable change was observed in the microvessels after the superfusion of 1 pM ET-3. Superfusion of 1 pM ET-3 with 100 μM L-NMMA elicited the vasoconstriction in arterioles, and the arteriolar diameter recovered to the control level within ten minutes in spite of continuing the superfusion. The vasoconstriction induced by low- dose ET-3 with L-NMMA was suppressed by the additional superfusion of 200 μM L-arginine. The present study suggests that the recovery of arteriolar diameter after the ET-3-induced constriction may be mediated by nitric oxide at least in the early phase.",
author = "I. Kurose and D. Fukumura and S. Miura and E. Sekizuka and H. Nagata and M. Suematsu and M. Tsuchiya",
year = "1993",
language = "English",
volume = "44",
pages = "483--490",
journal = "Angiology",
issn = "0003-3197",
publisher = "SAGE Publications Inc.",
number = "6",

}

TY - JOUR

T1 - Nitric oxide mediates vasoactive effects of endothelin-3 on rat mesenteric microvascular beds in vivo

AU - Kurose, I.

AU - Fukumura, D.

AU - Miura, S.

AU - Sekizuka, E.

AU - Nagata, H.

AU - Suematsu, M.

AU - Tsuchiya, M.

PY - 1993

Y1 - 1993

N2 - In order to clarify whether the vasoactive effects of endothelin-3 (ET-3) on microvessels are associated with the endothelium-derived relaxing factor (EDRF) in vivo, the authors examined the effects of L-N(G)-monomethyl arginine (L-NMMA), an analog of L-arginine, on low-dose ET-3 induced hemodynamic changes in the mesenteric microcirculation of male Wistar rats. The intravital observation revealed that ET-3 100 pM induced a remarkable and periodic vasoconstriction in arterioles, and the constriction was sustained for approximately fifteen minutes. No remarkable change was observed in the microvessels after the superfusion of 1 pM ET-3. Superfusion of 1 pM ET-3 with 100 μM L-NMMA elicited the vasoconstriction in arterioles, and the arteriolar diameter recovered to the control level within ten minutes in spite of continuing the superfusion. The vasoconstriction induced by low- dose ET-3 with L-NMMA was suppressed by the additional superfusion of 200 μM L-arginine. The present study suggests that the recovery of arteriolar diameter after the ET-3-induced constriction may be mediated by nitric oxide at least in the early phase.

AB - In order to clarify whether the vasoactive effects of endothelin-3 (ET-3) on microvessels are associated with the endothelium-derived relaxing factor (EDRF) in vivo, the authors examined the effects of L-N(G)-monomethyl arginine (L-NMMA), an analog of L-arginine, on low-dose ET-3 induced hemodynamic changes in the mesenteric microcirculation of male Wistar rats. The intravital observation revealed that ET-3 100 pM induced a remarkable and periodic vasoconstriction in arterioles, and the constriction was sustained for approximately fifteen minutes. No remarkable change was observed in the microvessels after the superfusion of 1 pM ET-3. Superfusion of 1 pM ET-3 with 100 μM L-NMMA elicited the vasoconstriction in arterioles, and the arteriolar diameter recovered to the control level within ten minutes in spite of continuing the superfusion. The vasoconstriction induced by low- dose ET-3 with L-NMMA was suppressed by the additional superfusion of 200 μM L-arginine. The present study suggests that the recovery of arteriolar diameter after the ET-3-induced constriction may be mediated by nitric oxide at least in the early phase.

UR - http://www.scopus.com/inward/record.url?scp=0027284875&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027284875&partnerID=8YFLogxK

M3 - Article

C2 - 8503515

AN - SCOPUS:0027284875

VL - 44

SP - 483

EP - 490

JO - Angiology

JF - Angiology

SN - 0003-3197

IS - 6

ER -