Nitrogen-doped carbon dots/Ni-MnFe-layered double hydroxides (N-CDs/Ni-MnFe-LDHs) hybrid nanomaterials as immunoassay label for low-density lipoprotein detection

Akarapong Prakobkij, Purim Jarujamrus, Suticha Chunta, Runglawan Chawengkirttikul, Tinnagon Keawin, Nutthaporn Malahom, Suparb Tamuang, Maliwan Amatathongchai, Daniel Citterio

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Nitrogen-doped carbon dots/Ni-MnFe-layered double hydroxides (N-CDs/Ni-MnFe-LDHs) are demonstrated as superior peroxidase mimic antibody labels alternative to horseradish peroxidase (HRP) in an immunoassay, potentially overcoming some of the inherent disadvantages of HRP and other enzyme mimicking nanomaterials. They revealed efficient peroxidase-like activity and catalyzed the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to form the intense blue product (at 620 nm) in the presence of hydrogen peroxide (H2O2). Using low-density lipoprotein (LDL) as a model target, an ultra-low limit of detection (0.0051 mg/dL) and a linear range of 0.0625–0.750 mg/dL were achieved, exhibiting higher sensitivity than the HRP-based immunoassay. Thus, the proposed N-CDs/Ni-MnFe-LDHs can be used as HRP mimicking analogs for developing highly sensitive colorimetric immunosensors for detection of biomarkers, as well as trace chemical analysis. Graphical abstract: [Figure not available: see fulltext.]

Original languageEnglish
Article number72
JournalMikrochimica Acta
Volume189
Issue number2
DOIs
Publication statusPublished - 2022 Feb

Keywords

  • Enzyme mimic
  • Immunoassay label
  • Low-density lipoprotein
  • Ni-MnFe-layered double hydroxides
  • Nitrogen-doped carbon dots

ASJC Scopus subject areas

  • Analytical Chemistry

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