NMR Characterization of the Conformations, Dynamics, and Interactions of Glycosphingolipids

Maho Yagi-Utsumi, Takumi Yamaguchi, Yoshinori Uekusa, Koichi Kato

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

Glycosphingolipids, including gangliosides, can form clusters on membranes and thereby mediate a variety of physiological and pathological functions. This chapter illustrates recent nuclear magnetic resonance (NMR) approaches for elucidating the molecular mechanisms underlying the functional roles of glycosphingolipid clusters, particularly focusing on the conformational dynamics and interactions of gangliosides. Dynamic conformational ensembles of ganglioside glycans in solution have been elucidated using molecular dynamics simulations validated by NMR data. Intermolecular interactions of glycosphingolipids in membrane-like environments have been characterized using solution and solid-state NMR techniques. Various membrane mimics have been developed for NMR studies on the interactions between ganglioside clusters and amyloidogenic proteins associated with neurodegenerative disorders.

Original languageEnglish
Title of host publicationBiophysics and Biochemistry of Cartilage by NMR and MRI
PublisherRoyal Society of Chemistry
Pages161-178
Number of pages18
Volume2017-January
Edition10
DOIs
Publication statusPublished - 2017
Externally publishedYes

Publication series

NameNew Developments in NMR
Number10
Volume2017-January
ISSN (Print)2044-253X
ISSN (Electronic)2044-2548

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ASJC Scopus subject areas

  • Analytical Chemistry
  • Radiology Nuclear Medicine and imaging
  • Spectroscopy

Cite this

Yagi-Utsumi, M., Yamaguchi, T., Uekusa, Y., & Kato, K. (2017). NMR Characterization of the Conformations, Dynamics, and Interactions of Glycosphingolipids. In Biophysics and Biochemistry of Cartilage by NMR and MRI (10 ed., Vol. 2017-January, pp. 161-178). (New Developments in NMR; Vol. 2017-January, No. 10). Royal Society of Chemistry. https://doi.org/10.1039/9781782623946-00161