NMR characterization of the interaction between the PUB domain of peptide: N-glycanase and ubiquitin-like domain of HR23

Yukiko Kamiya, Yoshinori Uekusa, Akira Sumiyoshi, Hiroaki Sasakawa, Takeshi Hirao, Tadashi Suzuki, Koichi Kato

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

PUB domains are identified in several proteins functioning in the ubiquitin (Ub)-proteasome system and considered as p97-binding modules. To address the further functional roles of these domains, we herein characterized the interactions of the PUB domain of peptide:N-glycanase (PNGase) with Ub and Ub-like domain (UBL) of the proteasome shuttle factor HR23. NMR data indicated that PNGase-PUB exerts an acceptor preferentially for HR23-UBL, electrostatically interacting with the UBL surface employed for binding to other Ub/UBL motifs. Our findings imply that PNGase-PUB serves not only as p97-binding module but also as a possible activator of HR23 in endoplasmic reticulum-associated degradation mechanisms. Structured summary of protein interactions: PNGase binds to HR23A by affinity chromatography technology (View interaction) PNGase and HR23A bind by nuclear magnetic resonance (View interaction) PNGase and HR23B bind by nuclear magnetic resonance (View interaction).

Original languageEnglish
Pages (from-to)1141-1146
Number of pages6
JournalFEBS Letters
Volume586
Issue number8
DOIs
Publication statusPublished - 2012 Apr 24
Externally publishedYes

Fingerprint

Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
Ubiquitin
Nuclear magnetic resonance
Proteasome Endopeptidase Complex
Magnetic Resonance Spectroscopy
Endoplasmic Reticulum-Associated Degradation
Affinity chromatography
Affinity Chromatography
Proteins
Technology
Degradation

Keywords

  • Endoplasmic reticulum-associated degradation
  • HR23
  • NMR
  • Peptide:N-glycanase
  • PUB domain
  • Ubiquitin-proteasome system

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

NMR characterization of the interaction between the PUB domain of peptide : N-glycanase and ubiquitin-like domain of HR23. / Kamiya, Yukiko; Uekusa, Yoshinori; Sumiyoshi, Akira; Sasakawa, Hiroaki; Hirao, Takeshi; Suzuki, Tadashi; Kato, Koichi.

In: FEBS Letters, Vol. 586, No. 8, 24.04.2012, p. 1141-1146.

Research output: Contribution to journalArticle

Kamiya, Yukiko ; Uekusa, Yoshinori ; Sumiyoshi, Akira ; Sasakawa, Hiroaki ; Hirao, Takeshi ; Suzuki, Tadashi ; Kato, Koichi. / NMR characterization of the interaction between the PUB domain of peptide : N-glycanase and ubiquitin-like domain of HR23. In: FEBS Letters. 2012 ; Vol. 586, No. 8. pp. 1141-1146.
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