NMR characterization of the interaction of GroEL with amyloid β as a model ligand

Maho Yagi-Utsumi, Tomoko Kunihara, Takashi Nakamura, Yoshinori Uekusa, Koki Makabe, Kunihiro Kuwajima, Koichi Kato

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Here we report an NMR study on the substrate interaction modes of GroEL using amyloid β (Aβ) as a model ligand. We found that GroEL could suppress Aβ(1-40) amyloid formation by interacting with its two hydrophobic segments Leu17-Ala21 and Ala30-Val36, which involve key residues in fibril formation. The binding site of Aβ(1-40) was mapped on a pair of α-helices located in the GroEL apical domain. These results provide insights into chaperonin recognition of amyloidogenic proteins of pathological interest.

Original languageEnglish
Pages (from-to)1605-1609
Number of pages5
JournalFEBS Letters
Volume587
Issue number11
DOIs
Publication statusPublished - 2013 Jun 5
Externally publishedYes

Fingerprint

Amyloid
Nuclear magnetic resonance
Chaperonins
Ligands
Amyloidogenic Proteins
Binding Sites
Substrates

Keywords

  • Amyloid β
  • Chaperonin
  • GroEL
  • NMR
  • NOE

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Yagi-Utsumi, M., Kunihara, T., Nakamura, T., Uekusa, Y., Makabe, K., Kuwajima, K., & Kato, K. (2013). NMR characterization of the interaction of GroEL with amyloid β as a model ligand. FEBS Letters, 587(11), 1605-1609. https://doi.org/10.1016/j.febslet.2013.04.007

NMR characterization of the interaction of GroEL with amyloid β as a model ligand. / Yagi-Utsumi, Maho; Kunihara, Tomoko; Nakamura, Takashi; Uekusa, Yoshinori; Makabe, Koki; Kuwajima, Kunihiro; Kato, Koichi.

In: FEBS Letters, Vol. 587, No. 11, 05.06.2013, p. 1605-1609.

Research output: Contribution to journalArticle

Yagi-Utsumi, M, Kunihara, T, Nakamura, T, Uekusa, Y, Makabe, K, Kuwajima, K & Kato, K 2013, 'NMR characterization of the interaction of GroEL with amyloid β as a model ligand', FEBS Letters, vol. 587, no. 11, pp. 1605-1609. https://doi.org/10.1016/j.febslet.2013.04.007
Yagi-Utsumi M, Kunihara T, Nakamura T, Uekusa Y, Makabe K, Kuwajima K et al. NMR characterization of the interaction of GroEL with amyloid β as a model ligand. FEBS Letters. 2013 Jun 5;587(11):1605-1609. https://doi.org/10.1016/j.febslet.2013.04.007
Yagi-Utsumi, Maho ; Kunihara, Tomoko ; Nakamura, Takashi ; Uekusa, Yoshinori ; Makabe, Koki ; Kuwajima, Kunihiro ; Kato, Koichi. / NMR characterization of the interaction of GroEL with amyloid β as a model ligand. In: FEBS Letters. 2013 ; Vol. 587, No. 11. pp. 1605-1609.
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