No involvement of IgG autoantibodies against extracellular domains of desmoglein 2 in paraneoplastic pemphigus or inflammatory bowel diseases

Takayuki Ota, Masayuki Amagai, Mamoru Watanabe, Takeji Nishikawa

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background: Patients with paraneoplastic pemphigus (PNP) and inflammatory bowel diseases, such as Crohn's disease (CD) and ulcerative colitis (UC), develop autoantibodies against simple epithelial cells. About 20-30% of patients with PNP develop fatal bronchiolitis obliterans, in which autoantibody-mediated injury is suspected because of in vivo IgG deposition on cell surfaces of bronchial epithelia. Objective: The purpose of this study is to determine whether patients with PNP, CD and UC have IgG autoantibodies against desmoglein 2 (Dsg2), which is expressed in all desmosome-bearing cells including respiratory and intestinal epithelia. Methods: A secreted form of recombinant Dsg2 (rDsg2-His) which contains its entire extracellular domains was produced by baculovirus expression. The reactivity of patients' sera against rDsg2-His was examined by ELISA as well as immunoprecipitation. Results: An anti-Dsg2 mouse monoclonal antibody, 6D8, showed positive reactivity against rDsg2-His in both methods. However, none of 38 PNP sera reacted with rDsg2-His by ELISA and none of 15 PNP sera tested immunoprecipitated rDsg2-His. Furthermore, none of 12 CD or 27 UC sera reacted with rDsg2-His by ELISA. Conclusion: These findings indicate that IgG autoantibodies against Dsg2 are not involved in PNP, CD or UC and suggest the existence of other unknown cell surface target antigen(s) in bronchiolitis obliterans in PNP.

Original languageEnglish
Pages (from-to)137-141
Number of pages5
JournalJournal of Dermatological Science
Volume32
Issue number2
DOIs
Publication statusPublished - 2003 Aug

Keywords

  • Autoimmune disease
  • Cadherin
  • Crohn's disease
  • Ulcerative colitis

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology

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