Abstract
Background: Patients with paraneoplastic pemphigus (PNP) and inflammatory bowel diseases, such as Crohn's disease (CD) and ulcerative colitis (UC), develop autoantibodies against simple epithelial cells. About 20-30% of patients with PNP develop fatal bronchiolitis obliterans, in which autoantibody-mediated injury is suspected because of in vivo IgG deposition on cell surfaces of bronchial epithelia. Objective: The purpose of this study is to determine whether patients with PNP, CD and UC have IgG autoantibodies against desmoglein 2 (Dsg2), which is expressed in all desmosome-bearing cells including respiratory and intestinal epithelia. Methods: A secreted form of recombinant Dsg2 (rDsg2-His) which contains its entire extracellular domains was produced by baculovirus expression. The reactivity of patients' sera against rDsg2-His was examined by ELISA as well as immunoprecipitation. Results: An anti-Dsg2 mouse monoclonal antibody, 6D8, showed positive reactivity against rDsg2-His in both methods. However, none of 38 PNP sera reacted with rDsg2-His by ELISA and none of 15 PNP sera tested immunoprecipitated rDsg2-His. Furthermore, none of 12 CD or 27 UC sera reacted with rDsg2-His by ELISA. Conclusion: These findings indicate that IgG autoantibodies against Dsg2 are not involved in PNP, CD or UC and suggest the existence of other unknown cell surface target antigen(s) in bronchiolitis obliterans in PNP.
| Original language | English |
|---|---|
| Pages (from-to) | 137-141 |
| Number of pages | 5 |
| Journal | Journal of Dermatological Science |
| Volume | 32 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2003 Aug |
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Keywords
- Autoimmune disease
- Cadherin
- Crohn's disease
- Ulcerative colitis
ASJC Scopus subject areas
- Dermatology
Cite this
No involvement of IgG autoantibodies against extracellular domains of desmoglein 2 in paraneoplastic pemphigus or inflammatory bowel diseases. / Ota, Takayuki; Amagai, Masayuki; Watanabe, Mamoru; Nishikawa, Takeji.
In: Journal of Dermatological Science, Vol. 32, No. 2, 08.2003, p. 137-141.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - No involvement of IgG autoantibodies against extracellular domains of desmoglein 2 in paraneoplastic pemphigus or inflammatory bowel diseases
AU - Ota, Takayuki
AU - Amagai, Masayuki
AU - Watanabe, Mamoru
AU - Nishikawa, Takeji
PY - 2003/8
Y1 - 2003/8
N2 - Background: Patients with paraneoplastic pemphigus (PNP) and inflammatory bowel diseases, such as Crohn's disease (CD) and ulcerative colitis (UC), develop autoantibodies against simple epithelial cells. About 20-30% of patients with PNP develop fatal bronchiolitis obliterans, in which autoantibody-mediated injury is suspected because of in vivo IgG deposition on cell surfaces of bronchial epithelia. Objective: The purpose of this study is to determine whether patients with PNP, CD and UC have IgG autoantibodies against desmoglein 2 (Dsg2), which is expressed in all desmosome-bearing cells including respiratory and intestinal epithelia. Methods: A secreted form of recombinant Dsg2 (rDsg2-His) which contains its entire extracellular domains was produced by baculovirus expression. The reactivity of patients' sera against rDsg2-His was examined by ELISA as well as immunoprecipitation. Results: An anti-Dsg2 mouse monoclonal antibody, 6D8, showed positive reactivity against rDsg2-His in both methods. However, none of 38 PNP sera reacted with rDsg2-His by ELISA and none of 15 PNP sera tested immunoprecipitated rDsg2-His. Furthermore, none of 12 CD or 27 UC sera reacted with rDsg2-His by ELISA. Conclusion: These findings indicate that IgG autoantibodies against Dsg2 are not involved in PNP, CD or UC and suggest the existence of other unknown cell surface target antigen(s) in bronchiolitis obliterans in PNP.
AB - Background: Patients with paraneoplastic pemphigus (PNP) and inflammatory bowel diseases, such as Crohn's disease (CD) and ulcerative colitis (UC), develop autoantibodies against simple epithelial cells. About 20-30% of patients with PNP develop fatal bronchiolitis obliterans, in which autoantibody-mediated injury is suspected because of in vivo IgG deposition on cell surfaces of bronchial epithelia. Objective: The purpose of this study is to determine whether patients with PNP, CD and UC have IgG autoantibodies against desmoglein 2 (Dsg2), which is expressed in all desmosome-bearing cells including respiratory and intestinal epithelia. Methods: A secreted form of recombinant Dsg2 (rDsg2-His) which contains its entire extracellular domains was produced by baculovirus expression. The reactivity of patients' sera against rDsg2-His was examined by ELISA as well as immunoprecipitation. Results: An anti-Dsg2 mouse monoclonal antibody, 6D8, showed positive reactivity against rDsg2-His in both methods. However, none of 38 PNP sera reacted with rDsg2-His by ELISA and none of 15 PNP sera tested immunoprecipitated rDsg2-His. Furthermore, none of 12 CD or 27 UC sera reacted with rDsg2-His by ELISA. Conclusion: These findings indicate that IgG autoantibodies against Dsg2 are not involved in PNP, CD or UC and suggest the existence of other unknown cell surface target antigen(s) in bronchiolitis obliterans in PNP.
KW - Autoimmune disease
KW - Cadherin
KW - Crohn's disease
KW - Ulcerative colitis
UR - http://www.scopus.com/inward/record.url?scp=0038500657&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0038500657&partnerID=8YFLogxK
U2 - 10.1016/S0923-1811(03)00072-0
DO - 10.1016/S0923-1811(03)00072-0
M3 - Article
C2 - 12850306
AN - SCOPUS:0038500657
VL - 32
SP - 137
EP - 141
JO - Journal of Dermatological Science
JF - Journal of Dermatological Science
SN - 0923-1811
IS - 2
ER -