NO/cGMP/PKG signaling pathway induces magnesium release mediated by mitoKATP channel opening in rat hippocampal neurons

Ryu Yamanaka, Yutaka Shindo, Kohji Hotta, Koji Suzuki, Kotaro Oka

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Intracellular Mg2+ concentration ([Mg2+]i) and NO regulate cell survival and death. To reveal the involvement of NO in intracellular Mg2+ regulation, we visualized intracellular Mg 2+ using the fluorescent Mg2+ indicator KMG-104-AM in rat hippocampal neurons. Pharmacological experiments using SNAP, 8-Br-cGMP, diazoxide and several inhibitors revealed that the NO/cGMP/Protein kinsase G (PKG) signaling pathway triggers an increase in [Mg2+]i, and that Mg2+ mobilization is due to Mg2+ release from mitochondria induced by mitoKATP channel opening. In addition, Mg2+ release is potentiated by the positive feedback loop including mitoKATP channel opening, mitochondrial depolarization and PKC activation.

Original languageEnglish
Pages (from-to)2643-2648
Number of pages6
JournalFEBS Letters
Volume587
Issue number16
DOIs
Publication statusPublished - 2013 Aug 19

Fingerprint

Magnesium
Neurons
Rats
Diazoxide
Mitochondria
Depolarization
Cell Survival
Proteins
Cell Death
Chemical activation
Cells
Pharmacology
Feedback
Experiments
mitochondrial K(ATP) channel

Keywords

  • Hippocampal neuron
  • KMG-104 mitoK channel
  • Mg
  • Mitochondria

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

NO/cGMP/PKG signaling pathway induces magnesium release mediated by mitoKATP channel opening in rat hippocampal neurons. / Yamanaka, Ryu; Shindo, Yutaka; Hotta, Kohji; Suzuki, Koji; Oka, Kotaro.

In: FEBS Letters, Vol. 587, No. 16, 19.08.2013, p. 2643-2648.

Research output: Contribution to journalArticle

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