NO/cGMP/PKG signaling pathway induces magnesium release mediated by mitoKATP channel opening in rat hippocampal neurons

Ryu Yamanaka, Yutaka Shindo, Kohji Hotta, Koji Suzuki, Kotaro Oka

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)


Intracellular Mg2+ concentration ([Mg2+]i) and NO regulate cell survival and death. To reveal the involvement of NO in intracellular Mg2+ regulation, we visualized intracellular Mg 2+ using the fluorescent Mg2+ indicator KMG-104-AM in rat hippocampal neurons. Pharmacological experiments using SNAP, 8-Br-cGMP, diazoxide and several inhibitors revealed that the NO/cGMP/Protein kinsase G (PKG) signaling pathway triggers an increase in [Mg2+]i, and that Mg2+ mobilization is due to Mg2+ release from mitochondria induced by mitoKATP channel opening. In addition, Mg2+ release is potentiated by the positive feedback loop including mitoKATP channel opening, mitochondrial depolarization and PKC activation.

Original languageEnglish
Pages (from-to)2643-2648
Number of pages6
JournalFEBS Letters
Issue number16
Publication statusPublished - 2013 Aug 19


  • Hippocampal neuron
  • KMG-104 mitoK channel
  • Mg
  • Mitochondria

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


Dive into the research topics of 'NO/cGMP/PKG signaling pathway induces magnesium release mediated by mitoK<sub>ATP</sub> channel opening in rat hippocampal neurons'. Together they form a unique fingerprint.

Cite this