NOD-Rag2null IL-2Rγnull mice: An alternative to NOG mice for generation of humanized mice

Ikumi Katano, Ryoji Ito, Tsutomu Kamisako, Tomoo Eto, Tomoyuki Ogura, Kenji Kawai, Hiroshi Suemizu, Takeshi Takahashi, Yutaka Kawakami, Mamoru Ito

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We have developed NOD-Rag2null IL-2Rγnull (NR2G) mice similar to NOD-scid IL-2Rγnull (NOG) mice that are known as an excellent host to generate humanized mice. To evaluate the usefulness of NR2G mice as a host for humanized mice, the engraftment rates and differentiation of human cells after human hematopoietic stem cell (HSC) transplantation were compared among NR2G, NOG, and NOD-scid mice. For this purpose, the appropriate irradiation doses to expand the niche for human stem cells in the bone marrow were first determined. As a result, 8 and 2.5 Gy in adult, and 4 and 1 Gy in newborn NR2G and NOG mice, respectively, were found to be appropriate. Next, 5 × 104 human umbilical cord blood CD34+ cells were intravenously inoculated into irradiated adult or newborn of the immunodeficient mice. These HSC transplantation experiments demonstrated that both NR2G and NOG mice showed high engraftment rates compared with NOD-scid mice, although NOG mice showed a slightly higher engraftment rate than that for NR2G mice. However, no difference was found in the human cell populations differentiated from HSCs between NR2G and NOG mice. The HSC transplantation experiments to adults and newborns of two immunodeficient mice also revealed that the HSC transplantation into newborn mice resulted in higher engraftment rate than those into adults. These results showed that NR2G mice could be used as an alternative host to NOG mice to generate humanized mice.

Original languageEnglish
Pages (from-to)321-330
Number of pages10
JournalExperimental Animals
Volume63
Issue number3
DOIs
Publication statusPublished - 2014

Keywords

  • Humanized mice
  • Immunodeficient mice
  • NOG mice

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Biochemistry, Genetics and Molecular Biology(all)
  • veterinary(all)

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    Katano, I., Ito, R., Kamisako, T., Eto, T., Ogura, T., Kawai, K., Suemizu, H., Takahashi, T., Kawakami, Y., & Ito, M. (2014). NOD-Rag2null IL-2Rγnull mice: An alternative to NOG mice for generation of humanized mice. Experimental Animals, 63(3), 321-330. https://doi.org/10.1538/expanim.63.321