TY - JOUR
T1 - Nodal/Bozozok-independent induction of the dorsal organizer by zebrafish cell lines
AU - Hashiguchi, Megumi
AU - Shinya, Minori
AU - Tokumoto, Mika
AU - Sakai, Noriyoshi
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2008/9/15
Y1 - 2008/9/15
N2 - Formation of the dorsal organizer (Spemann organizer) is an important process in early vertebrate development. In zebrafish, two molecular cascades-Bozozok/Dharma (Boz) and Nodal signaling-act in parallel to induce the dorsal organizer. However, the complete molecular mechanism regulating this event remains unclear. Here we report that zebrafish cell lines derived from various developmental stages can induce a secondary axis when they are implanted into the mid-blastula but not the early gastrula. The implanted cellsthemselves did not differentiate, but instead induced ectopic expression of dorsal organizer markers incells around the implanted cells and induced notochord formation in the secondary axis. These results indicate that cultured cell lines have the ability to induce a secondary axis through the initiation of dorsal organizer activity. However, ectopic expression of boz and sqt were not observed in cultured cells. In addition, implanted cell lines could induce the dorsal organizer even in maternal-zygotic one-eyed pinhead mutants, which are not responsive to Nodal signaling. Finally, the Nodal signaling pathway was not activatedfollowing implantation of cultured cells. Collectively, these data suggest that zebrafish cell lines induce the dorsal organizer independent of the boz and Nodal signaling pathways.
AB - Formation of the dorsal organizer (Spemann organizer) is an important process in early vertebrate development. In zebrafish, two molecular cascades-Bozozok/Dharma (Boz) and Nodal signaling-act in parallel to induce the dorsal organizer. However, the complete molecular mechanism regulating this event remains unclear. Here we report that zebrafish cell lines derived from various developmental stages can induce a secondary axis when they are implanted into the mid-blastula but not the early gastrula. The implanted cellsthemselves did not differentiate, but instead induced ectopic expression of dorsal organizer markers incells around the implanted cells and induced notochord formation in the secondary axis. These results indicate that cultured cell lines have the ability to induce a secondary axis through the initiation of dorsal organizer activity. However, ectopic expression of boz and sqt were not observed in cultured cells. In addition, implanted cell lines could induce the dorsal organizer even in maternal-zygotic one-eyed pinhead mutants, which are not responsive to Nodal signaling. Finally, the Nodal signaling pathway was not activatedfollowing implantation of cultured cells. Collectively, these data suggest that zebrafish cell lines induce the dorsal organizer independent of the boz and Nodal signaling pathways.
KW - Cultured cells
KW - Dorsal axis formation
KW - Dorsal organizer
KW - Nodal
KW - Zebrafish
UR - http://www.scopus.com/inward/record.url?scp=51249100203&partnerID=8YFLogxK
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U2 - 10.1016/j.ydbio.2008.06.035
DO - 10.1016/j.ydbio.2008.06.035
M3 - Article
C2 - 18652819
AN - SCOPUS:51249100203
VL - 321
SP - 387
EP - 396
JO - Developmental Biology
JF - Developmental Biology
SN - 0012-1606
IS - 2
ER -