Non-adherent cell-specific expression of DOCK2, a member of the human CDM-family proteins

Hiroshi Nishihara, Shin Kobayashi, Yuko Hashimoto, Fumiyo Ohba, Naoki Mochizuki, Takeshi Kurata, Kazuo Nagashima, Michiyuki Matsuda

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Human DOCK180, which was originally identified as a major protein bound to the Crk oncogene product, is an archetype of the CDM family of proteins, including Ced-5 of Caenorhabditis elegans and Mbc of Drosophila melanogaster. After DOCK180, at least three putative human proteins that manifest high amino acid sequence similarity to DOCK180 have been registered in the GenBank/EMBL database. We have designated one of them, KIAA0209, as DOCK2 and characterize here. DOCK2 mRNA was expressed mostly in peripheral blood cells, followed by slight expression in the spleen and thymus, whereas DOCK180 was expressed in all tissues tested except in peripheral blood cells. Immunostaining of human cadaver tissues revealed that the expression of DOCK2 was limited to the lymphocytes and macrophages of various organs. DOCK2 bound to and activated Rac1, as did DOCK180; however, DOCK2 did not bind to CrkII, which transduces signals at focal adhesions. Thus, DOCK180 and DOCK2 are regulators of Rac and function in adherent and non-adherent cells, respectively.

Original languageEnglish
Pages (from-to)179-187
Number of pages9
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1452
Issue number2
DOIs
Publication statusPublished - 1999 Nov 11
Externally publishedYes

Keywords

  • CDM-family protein
  • Ced-5
  • DOCK180
  • Mbc

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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