Non-invasive diagnosis of cutaneous leishmaniasis by the direct boil loop-mediated isothermal amplification method and MinION™ nanopore sequencing

Kazuo Imai; Norihito Tarumoto; Kiyoko Amo; Makoto Takahashi; Naoya Sakamoto; Atsushi Kosaka; Yasuyuki Kato; Kei Mikita; Jun Sakai; Takashi Murakami; Yutaka Suzuki; Shigefumi Maesaki; Takuya Maeda

doi:10.1016/j.parint.2017.03.001

Non-invasive diagnosis of cutaneous leishmaniasis by the direct boil loop-mediated isothermal amplification method and MinION™ nanopore sequencing

Kazuo Imai, Norihito Tarumoto, Kiyoko Amo, Makoto Takahashi, Naoya Sakamoto, Atsushi Kosaka, Yasuyuki Kato, Kei Mikita, Jun Sakai, Takashi Murakami, Yutaka Suzuki, Shigefumi Maesaki, Takuya Maeda

Department of Infectious Diseases

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Cutaneous leishmaniasis (CL) is gaining attention as a public health problem. We present two cases of CL imported from Syria and Venezuela in Japan. We diagnosed them as CL non-invasively by the direct boil loop-mediated isothermal amplification method and an innovative sequencing method using the MinION™ sequencer. This report demonstrates that our procedure could be useful for the diagnosis of CL in both clinical and epidemiological settings.

Original language	English
Pages (from-to)	34-37
Number of pages	4
Journal	Parasitology International
Volume	67
Issue number	1
DOIs	https://doi.org/10.1016/j.parint.2017.03.001
Publication status	Published - 2018 Feb

Keywords

Cutaneous leishmaniasis;
Diagnosis
LAMP;
Leishmania braziliensis
Leishmania tropica
MinION™

ASJC Scopus subject areas

Parasitology
Infectious Diseases

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Imai, K., Tarumoto, N., Amo, K., Takahashi, M., Sakamoto, N., Kosaka, A., Kato, Y., Mikita, K., Sakai, J., Murakami, T., Suzuki, Y., Maesaki, S., & Maeda, T. (2018). Non-invasive diagnosis of cutaneous leishmaniasis by the direct boil loop-mediated isothermal amplification method and MinION™ nanopore sequencing. Parasitology International, 67(1), 34-37. https://doi.org/10.1016/j.parint.2017.03.001

Non-invasive diagnosis of cutaneous leishmaniasis by the direct boil loop-mediated isothermal amplification method and MinION™ nanopore sequencing. / Imai, Kazuo; Tarumoto, Norihito; Amo, Kiyoko; Takahashi, Makoto; Sakamoto, Naoya; Kosaka, Atsushi; Kato, Yasuyuki; Mikita, Kei; Sakai, Jun; Murakami, Takashi; Suzuki, Yutaka; Maesaki, Shigefumi; Maeda, Takuya.

In: Parasitology International, Vol. 67, No. 1, 02.2018, p. 34-37.

Research output: Contribution to journal › Article › peer-review

Imai, K, Tarumoto, N, Amo, K, Takahashi, M, Sakamoto, N, Kosaka, A, Kato, Y, Mikita, K, Sakai, J, Murakami, T, Suzuki, Y, Maesaki, S & Maeda, T 2018, 'Non-invasive diagnosis of cutaneous leishmaniasis by the direct boil loop-mediated isothermal amplification method and MinION™ nanopore sequencing', Parasitology International, vol. 67, no. 1, pp. 34-37. https://doi.org/10.1016/j.parint.2017.03.001

Imai, Kazuo ; Tarumoto, Norihito ; Amo, Kiyoko ; Takahashi, Makoto ; Sakamoto, Naoya ; Kosaka, Atsushi ; Kato, Yasuyuki ; Mikita, Kei ; Sakai, Jun ; Murakami, Takashi ; Suzuki, Yutaka ; Maesaki, Shigefumi ; Maeda, Takuya. / Non-invasive diagnosis of cutaneous leishmaniasis by the direct boil loop-mediated isothermal amplification method and MinION™ nanopore sequencing. In: Parasitology International. 2018 ; Vol. 67, No. 1. pp. 34-37.

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