Non-invasive diagnosis of cutaneous leishmaniasis by the direct boil loop-mediated isothermal amplification method and MinION™ nanopore sequencing

Kazuo Imai, Norihito Tarumoto, Kiyoko Amo, Makoto Takahashi, Naoya Sakamoto, Atsushi Kosaka, Yasuyuki Kato, Kei Mikita, Jun Sakai, Takashi Murakami, Yutaka Suzuki, Shigefumi Maesaki, Takuya Maeda

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Cutaneous leishmaniasis (CL) is gaining attention as a public health problem. We present two cases of CL imported from Syria and Venezuela in Japan. We diagnosed them as CL non-invasively by the direct boil loop-mediated isothermal amplification method and an innovative sequencing method using the MinION™ sequencer. This report demonstrates that our procedure could be useful for the diagnosis of CL in both clinical and epidemiological settings.

Original languageEnglish
JournalParasitology International
DOIs
Publication statusAccepted/In press - 2017 Feb 28

Fingerprint

Nanopores
Furunculosis
Cutaneous Leishmaniasis
Syria
Venezuela
Japan
Public Health

Keywords

  • Cutaneous leishmaniasis;
  • Diagnosis
  • LAMP;
  • Leishmania braziliensis
  • Leishmania tropica
  • MinION™

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Non-invasive diagnosis of cutaneous leishmaniasis by the direct boil loop-mediated isothermal amplification method and MinION™ nanopore sequencing. / Imai, Kazuo; Tarumoto, Norihito; Amo, Kiyoko; Takahashi, Makoto; Sakamoto, Naoya; Kosaka, Atsushi; Kato, Yasuyuki; Mikita, Kei; Sakai, Jun; Murakami, Takashi; Suzuki, Yutaka; Maesaki, Shigefumi; Maeda, Takuya.

In: Parasitology International, 28.02.2017.

Research output: Contribution to journalArticle

Imai, Kazuo ; Tarumoto, Norihito ; Amo, Kiyoko ; Takahashi, Makoto ; Sakamoto, Naoya ; Kosaka, Atsushi ; Kato, Yasuyuki ; Mikita, Kei ; Sakai, Jun ; Murakami, Takashi ; Suzuki, Yutaka ; Maesaki, Shigefumi ; Maeda, Takuya. / Non-invasive diagnosis of cutaneous leishmaniasis by the direct boil loop-mediated isothermal amplification method and MinION™ nanopore sequencing. In: Parasitology International. 2017.
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AU - Sakamoto, Naoya

AU - Kosaka, Atsushi

AU - Kato, Yasuyuki

AU - Mikita, Kei

AU - Sakai, Jun

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AU - Suzuki, Yutaka

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