Non-invasive diagnosis of cutaneous leishmaniasis by the direct boil loop-mediated isothermal amplification method and MinION™ nanopore sequencing

Kazuo Imai, Norihito Tarumoto, Kiyoko Amo, Makoto Takahashi, Naoya Sakamoto, Atsushi Kosaka, Yasuyuki Kato, Kei Mikita, Jun Sakai, Takashi Murakami, Yutaka Suzuki, Shigefumi Maesaki, Takuya Maeda

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Cutaneous leishmaniasis (CL) is gaining attention as a public health problem. We present two cases of CL imported from Syria and Venezuela in Japan. We diagnosed them as CL non-invasively by the direct boil loop-mediated isothermal amplification method and an innovative sequencing method using the MinION™ sequencer. This report demonstrates that our procedure could be useful for the diagnosis of CL in both clinical and epidemiological settings.

Original languageEnglish
Pages (from-to)34-37
Number of pages4
JournalParasitology International
Volume67
Issue number1
DOIs
Publication statusPublished - 2018 Feb

Keywords

  • Cutaneous leishmaniasis;
  • Diagnosis
  • LAMP;
  • Leishmania braziliensis
  • Leishmania tropica
  • MinION™

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

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    Imai, K., Tarumoto, N., Amo, K., Takahashi, M., Sakamoto, N., Kosaka, A., Kato, Y., Mikita, K., Sakai, J., Murakami, T., Suzuki, Y., Maesaki, S., & Maeda, T. (2018). Non-invasive diagnosis of cutaneous leishmaniasis by the direct boil loop-mediated isothermal amplification method and MinION™ nanopore sequencing. Parasitology International, 67(1), 34-37. https://doi.org/10.1016/j.parint.2017.03.001