Non-invasive diagnosis of cutaneous leishmaniasis by the direct boil loop-mediated isothermal amplification method and MinION™ nanopore sequencing

Kazuo Imai; Norihito Tarumoto; Kiyoko Amo; Makoto Takahashi; Naoya Sakamoto; Atsushi Kosaka; Yasuyuki Kato; Kei Mikita; Jun Sakai; Takashi Murakami; Yutaka Suzuki; Shigefumi Maesaki; Takuya Maeda

doi:10.1016/j.parint.2017.03.001

Non-invasive diagnosis of cutaneous leishmaniasis by the direct boil loop-mediated isothermal amplification method and MinION™ nanopore sequencing

Kazuo Imai, Norihito Tarumoto, Kiyoko Amo, Makoto Takahashi, Naoya Sakamoto, Atsushi Kosaka, Yasuyuki Kato, Kei Mikita, Jun Sakai, Takashi Murakami, Yutaka Suzuki, Shigefumi Maesaki, Takuya Maeda

Department of Infectious Diseases

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

Cutaneous leishmaniasis (CL) is gaining attention as a public health problem. We present two cases of CL imported from Syria and Venezuela in Japan. We diagnosed them as CL non-invasively by the direct boil loop-mediated isothermal amplification method and an innovative sequencing method using the MinION™ sequencer. This report demonstrates that our procedure could be useful for the diagnosis of CL in both clinical and epidemiological settings.

Original language	English
Pages (from-to)	34-37
Number of pages	4
Journal	Parasitology International
Volume	67
Issue number	1
DOIs	https://doi.org/10.1016/j.parint.2017.03.001
Publication status	Published - 2018 Feb

Keywords

Cutaneous leishmaniasis;
Diagnosis
LAMP;
Leishmania braziliensis
Leishmania tropica
MinION™

ASJC Scopus subject areas

Parasitology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.parint.2017.03.001

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Imai, K., Tarumoto, N., Amo, K., Takahashi, M., Sakamoto, N., Kosaka, A., Kato, Y., Mikita, K., Sakai, J., Murakami, T., Suzuki, Y., Maesaki, S., & Maeda, T. (2018). Non-invasive diagnosis of cutaneous leishmaniasis by the direct boil loop-mediated isothermal amplification method and MinION™ nanopore sequencing. Parasitology International, 67(1), 34-37. https://doi.org/10.1016/j.parint.2017.03.001

Imai, K, Tarumoto, N, Amo, K, Takahashi, M, Sakamoto, N, Kosaka, A, Kato, Y, Mikita, K, Sakai, J, Murakami, T, Suzuki, Y, Maesaki, S & Maeda, T 2018, 'Non-invasive diagnosis of cutaneous leishmaniasis by the direct boil loop-mediated isothermal amplification method and MinION™ nanopore sequencing', Parasitology International, vol. 67, no. 1, pp. 34-37. https://doi.org/10.1016/j.parint.2017.03.001

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abstract = "Cutaneous leishmaniasis (CL) is gaining attention as a public health problem. We present two cases of CL imported from Syria and Venezuela in Japan. We diagnosed them as CL non-invasively by the direct boil loop-mediated isothermal amplification method and an innovative sequencing method using the MinION{\texttrademark} sequencer. This report demonstrates that our procedure could be useful for the diagnosis of CL in both clinical and epidemiological settings.",

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doi = "10.1016/j.parint.2017.03.001",

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T1 - Non-invasive diagnosis of cutaneous leishmaniasis by the direct boil loop-mediated isothermal amplification method and MinION™ nanopore sequencing

AU - Imai, Kazuo

AU - Tarumoto, Norihito

AU - Amo, Kiyoko

AU - Takahashi, Makoto

AU - Sakamoto, Naoya

AU - Kosaka, Atsushi

AU - Kato, Yasuyuki

AU - Mikita, Kei

AU - Sakai, Jun

AU - Murakami, Takashi

AU - Suzuki, Yutaka

AU - Maesaki, Shigefumi

AU - Maeda, Takuya

PY - 2018/2

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AB - Cutaneous leishmaniasis (CL) is gaining attention as a public health problem. We present two cases of CL imported from Syria and Venezuela in Japan. We diagnosed them as CL non-invasively by the direct boil loop-mediated isothermal amplification method and an innovative sequencing method using the MinION™ sequencer. This report demonstrates that our procedure could be useful for the diagnosis of CL in both clinical and epidemiological settings.

KW - Cutaneous leishmaniasis;

KW - Diagnosis

KW - LAMP;

KW - Leishmania braziliensis

KW - Leishmania tropica

KW - MinION™

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Non-invasive diagnosis of cutaneous leishmaniasis by the direct boil loop-mediated isothermal amplification method and MinION™ nanopore sequencing

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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