Non-invasive diagnosis of cutaneous leishmaniasis by the direct boil loop-mediated isothermal amplification method and MinION™ nanopore sequencing

Kazuo Imai, Norihito Tarumoto, Kiyoko Amo, Makoto Takahashi, Naoya Sakamoto, Atsushi Kosaka, Yasuyuki Kato, Kei Mikita, Jun Sakai, Takashi Murakami, Yutaka Suzuki, Shigefumi Maesaki, Takuya Maeda

Research output: Contribution to journal › Article

7 Citations (Scopus)

Abstract

Cutaneous leishmaniasis (CL) is gaining attention as a public health problem. We present two cases of CL imported from Syria and Venezuela in Japan. We diagnosed them as CL non-invasively by the direct boil loop-mediated isothermal amplification method and an innovative sequencing method using the MinION™ sequencer. This report demonstrates that our procedure could be useful for the diagnosis of CL in both clinical and epidemiological settings.

Original languageEnglish
JournalParasitology International
DOIs
Publication statusAccepted/In press - 2017 Feb 28

Fingerprint

Nanopores
Furunculosis
Cutaneous Leishmaniasis
Syria
Venezuela
Japan
Public Health

Keywords

  • Cutaneous leishmaniasis;
  • Diagnosis
  • LAMP;
  • Leishmania braziliensis
  • Leishmania tropica
  • MinION™

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Non-invasive diagnosis of cutaneous leishmaniasis by the direct boil loop-mediated isothermal amplification method and MinION™ nanopore sequencing. / Imai, Kazuo; Tarumoto, Norihito; Amo, Kiyoko; Takahashi, Makoto; Sakamoto, Naoya; Kosaka, Atsushi; Kato, Yasuyuki; Mikita, Kei; Sakai, Jun; Murakami, Takashi; Suzuki, Yutaka; Maesaki, Shigefumi; Maeda, Takuya.

In: Parasitology International, 28.02.2017.

Research output: Contribution to journal › Article

Imai, K, Tarumoto, N, Amo, K, Takahashi, M, Sakamoto, N, Kosaka, A, Kato, Y, Mikita, K, Sakai, J, Murakami, T, Suzuki, Y, Maesaki, S & Maeda, T 2017, 'Non-invasive diagnosis of cutaneous leishmaniasis by the direct boil loop-mediated isothermal amplification method and MinION™ nanopore sequencing', Parasitology International. https://doi.org/10.1016/j.parint.2017.03.001
Imai K, Tarumoto N, Amo K, Takahashi M, Sakamoto N, Kosaka A et al. Non-invasive diagnosis of cutaneous leishmaniasis by the direct boil loop-mediated isothermal amplification method and MinION™ nanopore sequencing. Parasitology International. 2017 Feb 28. https://doi.org/10.1016/j.parint.2017.03.001
Imai, Kazuo ; Tarumoto, Norihito ; Amo, Kiyoko ; Takahashi, Makoto ; Sakamoto, Naoya ; Kosaka, Atsushi ; Kato, Yasuyuki ; Mikita, Kei ; Sakai, Jun ; Murakami, Takashi ; Suzuki, Yutaka ; Maesaki, Shigefumi ; Maeda, Takuya. / Non-invasive diagnosis of cutaneous leishmaniasis by the direct boil loop-mediated isothermal amplification method and MinION™ nanopore sequencing. In: Parasitology International. 2017.
@article{619613008f49473aabd017829ed3e7c3,
title = "Non-invasive diagnosis of cutaneous leishmaniasis by the direct boil loop-mediated isothermal amplification method and MinION™ nanopore sequencing",
abstract = "Cutaneous leishmaniasis (CL) is gaining attention as a public health problem. We present two cases of CL imported from Syria and Venezuela in Japan. We diagnosed them as CL non-invasively by the direct boil loop-mediated isothermal amplification method and an innovative sequencing method using the MinION™ sequencer. This report demonstrates that our procedure could be useful for the diagnosis of CL in both clinical and epidemiological settings.",
keywords = "Cutaneous leishmaniasis;, Diagnosis, LAMP;, Leishmania braziliensis, Leishmania tropica, MinION™",
author = "Kazuo Imai and Norihito Tarumoto and Kiyoko Amo and Makoto Takahashi and Naoya Sakamoto and Atsushi Kosaka and Yasuyuki Kato and Kei Mikita and Jun Sakai and Takashi Murakami and Yutaka Suzuki and Shigefumi Maesaki and Takuya Maeda",
year = "2017",
month = "2",
day = "28",
doi = "10.1016/j.parint.2017.03.001",
language = "English",
journal = "Parasitology International",
issn = "1383-5769",
publisher = "Elsevier Ireland Ltd",

}

TY - JOUR

T1 - Non-invasive diagnosis of cutaneous leishmaniasis by the direct boil loop-mediated isothermal amplification method and MinION™ nanopore sequencing

AU - Imai, Kazuo

AU - Tarumoto, Norihito

AU - Amo, Kiyoko

AU - Takahashi, Makoto

AU - Sakamoto, Naoya

AU - Kosaka, Atsushi

AU - Kato, Yasuyuki

AU - Mikita, Kei

AU - Sakai, Jun

AU - Murakami, Takashi

AU - Suzuki, Yutaka

AU - Maesaki, Shigefumi

AU - Maeda, Takuya

PY - 2017/2/28

Y1 - 2017/2/28

N2 - Cutaneous leishmaniasis (CL) is gaining attention as a public health problem. We present two cases of CL imported from Syria and Venezuela in Japan. We diagnosed them as CL non-invasively by the direct boil loop-mediated isothermal amplification method and an innovative sequencing method using the MinION™ sequencer. This report demonstrates that our procedure could be useful for the diagnosis of CL in both clinical and epidemiological settings.

AB - Cutaneous leishmaniasis (CL) is gaining attention as a public health problem. We present two cases of CL imported from Syria and Venezuela in Japan. We diagnosed them as CL non-invasively by the direct boil loop-mediated isothermal amplification method and an innovative sequencing method using the MinION™ sequencer. This report demonstrates that our procedure could be useful for the diagnosis of CL in both clinical and epidemiological settings.

KW - Cutaneous leishmaniasis;

KW - Diagnosis

KW - LAMP;

KW - Leishmania braziliensis

KW - Leishmania tropica

KW - MinION™

UR - http://www.scopus.com/inward/record.url?scp=85015448924&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85015448924&partnerID=8YFLogxK

U2 - 10.1016/j.parint.2017.03.001

DO - 10.1016/j.parint.2017.03.001

M3 - Article

C2 - 28288843

AN - SCOPUS:85015448924

JO - Parasitology International

JF - Parasitology International

SN - 1383-5769

ER -

Access to Document