Non-membranous granular organelle consisting of PCM-1: Subcellular distribution and cell-cycle-dependent assembly/disassembly

Akiharu Kubo, Shoichiro Tsukita

Research output: Contribution to journalReview article

77 Citations (Scopus)

Abstract

Centriolar satellites were initially identified as electron-dense spherical granules, ∼70-100 nm in diameter, localized around the centrosomes. We have previously identified pericentriolar material 1 (PCM-1), with a molecular mass of ∼230 kDa, as a component of centriolar satellites. We now show by immunofluorescence microscopy that these granules are not only concentrated around centrioles but also scattered throughout the cytoplasm in various types of mouse cells, leading us tentatively to call them 'PCM-1 granules'. We then found that, when overexpressed, PCM-1 molecules lacking their C-terminal region bound directly with each other through two distinct regions to form large aggregates, which then recruited endogenous PCM-1. These large aggregates as well as endogenous PCM-1 granules appear to be disassembled during mitosis, and re-assembled when the cells entered interphase. These findings suggest that PCM-1 granules are formed by self-aggregation of PCM-1 and that this self-aggregation is regulated in a cell-cycle-dependent manner. Furthermore, we found that PCM-1 granules are distinct from pericentrin-containing granules, and that these two distinct types of granular structures are frequently associated with each other within the cytoplasm. These findings are discussed with special reference to the possible physiological functions of PCM-1 granules.

Original languageEnglish
Pages (from-to)919-928
Number of pages10
JournalJournal of Cell Science
Volume116
Issue number5
DOIs
Publication statusPublished - 2003 Mar 1
Externally publishedYes

Keywords

  • Centriolar satellites
  • Centrosome
  • PCM-1
  • Pericentrin
  • γ-Tubulin

ASJC Scopus subject areas

  • Cell Biology

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