Non-neuronal, but atropine-sensitive ileal contractile responses to short-chain fatty acids

Age-dependent desensitization and restoration under inflammatory conditions in mice

Masako Yajima, Shunsuke Kimura, Shinichiro Karaki, Junko Nio-Kobayashi, Takeshi Tsuruta, Atsukazu Kuwahara, Takaji Yajima, Toshihiko Iwanaga

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Intestinal epithelial cells sense short-chain fatty acids (SCFAs) to secrete non-neuronal acetylcholine (ACh). However, the roles of luminal SCFAs and epithelial ACh under normal and pathological conditions remain unknown. We examined ileal contractile responses to SCFAs at different ages and their mucosal cholinergic alterations under inflammatory conditions. Ileal contractile responses to SCFAs in 1-day-old pups to 7-week-old mice were compared using an isotonic transducer, and responses to an intraperitoneal injection of lipopolysaccharide (LPS) were analyzed in 7-week-old mice. The mRNA expression levels of a SCFA activate free fatty acid receptor, acetylcholinesterase (AChE), choline acetyltransferase (Chat), and choline transporter-like protein 4 (CTL4) were measured using real-time quantitative RT-PCR. AChE was analyzed by histochemical and optical enzymatic assays. Atropine-sensitive ileal contractile responses to SCFAs occurred in all 1-day-old pups, but were frequently desensitized after the weaning period. These contractile responses were not inhibited by tetrodotoxin and did not appear when the mucosal layer had been scraped off. Contractile desensitization in 7-week-old mice was abolished in the presence of the AChE inhibitor, eserine, which was consistent with increased AChE activity after weaning. Ileal contractions to SCFAs in adult mice were restored by LPS, which significantly increased the epithelial mRNA expression of Chat and CTL4. Atropine-sensitive ileal contractile responses to SCFAs constitutively occur in the newborn period, and are desensitized during developmental stages following the up-regulated expression of AChE in the villous mucosa, but are restored under inflammatory conditions possibly via the release of epithelial ACh.

Original languageEnglish
Pages (from-to)1-11
Number of pages11
JournalPhysiological Reports
Volume4
Issue number7
DOIs
Publication statusPublished - 2016 Apr 1
Externally publishedYes

Fingerprint

Volatile Fatty Acids
Atropine
Acetylcholinesterase
Acetylcholine
Choline O-Acetyltransferase
Weaning
Lipopolysaccharides
Messenger RNA
Physostigmine
Cholinesterase Inhibitors
Tetrodotoxin
Enzyme Assays
Intraperitoneal Injections
Transducers
Nonesterified Fatty Acids
Cholinergic Agents
Real-Time Polymerase Chain Reaction
Mucous Membrane
Proteins
Epithelial Cells

Keywords

  • Acetylcholinesterase
  • Age
  • Ileal contraction
  • Inflammatory condition
  • Non-neuronal acetylcholine
  • Short-chain fatty acids

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Non-neuronal, but atropine-sensitive ileal contractile responses to short-chain fatty acids : Age-dependent desensitization and restoration under inflammatory conditions in mice. / Yajima, Masako; Kimura, Shunsuke; Karaki, Shinichiro; Nio-Kobayashi, Junko; Tsuruta, Takeshi; Kuwahara, Atsukazu; Yajima, Takaji; Iwanaga, Toshihiko.

In: Physiological Reports, Vol. 4, No. 7, 01.04.2016, p. 1-11.

Research output: Contribution to journalArticle

Yajima, Masako ; Kimura, Shunsuke ; Karaki, Shinichiro ; Nio-Kobayashi, Junko ; Tsuruta, Takeshi ; Kuwahara, Atsukazu ; Yajima, Takaji ; Iwanaga, Toshihiko. / Non-neuronal, but atropine-sensitive ileal contractile responses to short-chain fatty acids : Age-dependent desensitization and restoration under inflammatory conditions in mice. In: Physiological Reports. 2016 ; Vol. 4, No. 7. pp. 1-11.
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