Non-spherical liposome formation using 3D-laser-printed micro-cube structures

K. Inoue, K. Kamiya, T. Osaki, Norihisa Miki, S. Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This conference paper describes a non-spherical liposome formation method using three-dimensional micro structures to emulate cytoskeleton proteins into biological cells. First, we fabricated a micro-cubic frame based on a stackable photoresist processed by direct laser writing. Next, we formed water-oil-water interfaces containing lipids on the faces of these microstructures in microfluidics channel. When the oil layer was sufficiently thin, lipid bilayer membranes (BLM) were formed. Moreover, we confirmed the BLM formation by reconstituting a membrane protein (alpha-hemolysin; αHL) and observed the diffusion of fluorescent molecules through the nanopores formed by αHL. We successfully formed the non-spherical liposomes with artificial cytoskeletons. The non-spherical liposomes will create new opportunities for the development of an artificial cell research model.

Original languageEnglish
Title of host publicationMEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages197-199
Number of pages3
Volume2016-February
ISBN (Electronic)9781509019731
DOIs
Publication statusPublished - 2016 Feb 26
Event29th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2016 - Shanghai, China
Duration: 2016 Jan 242016 Jan 28

Other

Other29th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2016
CountryChina
CityShanghai
Period16/1/2416/1/28

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials

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  • Cite this

    Inoue, K., Kamiya, K., Osaki, T., Miki, N., & Takeuchi, S. (2016). Non-spherical liposome formation using 3D-laser-printed micro-cube structures. In MEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems (Vol. 2016-February, pp. 197-199). [7421592] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMSYS.2016.7421592