Non-spherical liposome formation using 3D-laser-printed micro-cube structures

K. Inoue; K. Kamiya; T. Osaki; Norihisa Miki; S. Takeuchi

doi:10.1109/MEMSYS.2016.7421592

Non-spherical liposome formation using 3D-laser-printed micro-cube structures

K. Inoue, K. Kamiya, T. Osaki, Norihisa Miki, S. Takeuchi

Department of Mechanical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

This conference paper describes a non-spherical liposome formation method using three-dimensional micro structures to emulate cytoskeleton proteins into biological cells. First, we fabricated a micro-cubic frame based on a stackable photoresist processed by direct laser writing. Next, we formed water-oil-water interfaces containing lipids on the faces of these microstructures in microfluidics channel. When the oil layer was sufficiently thin, lipid bilayer membranes (BLM) were formed. Moreover, we confirmed the BLM formation by reconstituting a membrane protein (alpha-hemolysin; αHL) and observed the diffusion of fluorescent molecules through the nanopores formed by αHL. We successfully formed the non-spherical liposomes with artificial cytoskeletons. The non-spherical liposomes will create new opportunities for the development of an artificial cell research model.

Original language	English
Title of host publication	MEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	197-199
Number of pages	3
Volume	2016-February
ISBN (Electronic)	9781509019731
DOIs	https://doi.org/10.1109/MEMSYS.2016.7421592
Publication status	Published - 2016 Feb 26
Event	29th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2016 - Shanghai, China Duration: 2016 Jan 24 → 2016 Jan 28

Other

Other	29th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2016
Country	China
City	Shanghai
Period	16/1/24 → 16/1/28

ASJC Scopus subject areas

Electrical and Electronic Engineering
Mechanical Engineering
Condensed Matter Physics
Electronic, Optical and Magnetic Materials

Access to Document

10.1109/MEMSYS.2016.7421592

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Cite this

Inoue, K., Kamiya, K., Osaki, T., Miki, N., & Takeuchi, S. (2016). Non-spherical liposome formation using 3D-laser-printed micro-cube structures. In MEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems (Vol. 2016-February, pp. 197-199). [7421592] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMSYS.2016.7421592

Non-spherical liposome formation using 3D-laser-printed micro-cube structures. / Inoue, K.; Kamiya, K.; Osaki, T.; Miki, Norihisa; Takeuchi, S.

MEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems. Vol. 2016-February Institute of Electrical and Electronics Engineers Inc., 2016. p. 197-199 7421592.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Inoue, K, Kamiya, K, Osaki, T, Miki, N & Takeuchi, S 2016, Non-spherical liposome formation using 3D-laser-printed micro-cube structures. in MEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems. vol. 2016-February, 7421592, Institute of Electrical and Electronics Engineers Inc., pp. 197-199, 29th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2016, Shanghai, China, 16/1/24. https://doi.org/10.1109/MEMSYS.2016.7421592

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