TY - GEN
T1 - Non-spherical liposome formation using 3D-laser-printed micro-cube structures
AU - Inoue, K.
AU - Kamiya, K.
AU - Osaki, T.
AU - Miki, N.
AU - Takeuchi, S.
N1 - Publisher Copyright:
© 2016 IEEE.
Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 2016/2/26
Y1 - 2016/2/26
N2 - This conference paper describes a non-spherical liposome formation method using three-dimensional micro structures to emulate cytoskeleton proteins into biological cells. First, we fabricated a micro-cubic frame based on a stackable photoresist processed by direct laser writing. Next, we formed water-oil-water interfaces containing lipids on the faces of these microstructures in microfluidics channel. When the oil layer was sufficiently thin, lipid bilayer membranes (BLM) were formed. Moreover, we confirmed the BLM formation by reconstituting a membrane protein (alpha-hemolysin; αHL) and observed the diffusion of fluorescent molecules through the nanopores formed by αHL. We successfully formed the non-spherical liposomes with artificial cytoskeletons. The non-spherical liposomes will create new opportunities for the development of an artificial cell research model.
AB - This conference paper describes a non-spherical liposome formation method using three-dimensional micro structures to emulate cytoskeleton proteins into biological cells. First, we fabricated a micro-cubic frame based on a stackable photoresist processed by direct laser writing. Next, we formed water-oil-water interfaces containing lipids on the faces of these microstructures in microfluidics channel. When the oil layer was sufficiently thin, lipid bilayer membranes (BLM) were formed. Moreover, we confirmed the BLM formation by reconstituting a membrane protein (alpha-hemolysin; αHL) and observed the diffusion of fluorescent molecules through the nanopores formed by αHL. We successfully formed the non-spherical liposomes with artificial cytoskeletons. The non-spherical liposomes will create new opportunities for the development of an artificial cell research model.
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U2 - 10.1109/MEMSYS.2016.7421592
DO - 10.1109/MEMSYS.2016.7421592
M3 - Conference contribution
AN - SCOPUS:84971016646
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 197
EP - 199
BT - MEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 29th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2016
Y2 - 24 January 2016 through 28 January 2016
ER -