Non-viral derivation of a transgene-free induced pluripotent stem cell line from a male beagle dog

Sho Yoshimatsu, Kazuya Edamura, Yumi Yoshii, Aozora Iguchi, Hirotaka Kondo, Hisashi Shibuya, Tsukika Sato, Seiji Shiozawa, Hideyuki Okano

Research output: Contribution to journalArticlepeer-review

Abstract

We previously reported the non-viral derivation of transgene-free induced pluripotent stem cells (iPSCs) from somatic fibroblasts of a female beagle dog using an optimized induction medium and integration-free episomal vectors. Here, we report novel derivation of a male canine iPSC line OF35Y-iPS, which showed standard characteristics of pluripotency such as a strong gene expression profile of pluripotency markers, differentiation potential into all three germ layers, and normal karyotype (78XY). Furthermore, we demonstrated targeted integration of 2A-EGFP into the canine NANOS3 locus. The novel iPSC line would be a useful resource for stem cell research and regenerative veterinary medicine.

Original languageEnglish
Article number102375
JournalStem Cell Research
Volume53
DOIs
Publication statusPublished - 2021 May

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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