Nongenetic method for purifying stem cell-derived cardiomyocytes

Fumiyuki Hattori, Hao Chen, Hiromi Yamashita, Shugo Tohyama, Yu Suke Satoh, Shinsuke Yuasa, Weizhen Li, Hiroyuki Yamakawa, Tomofumi Tanaka, Takeshi Onitsuka, Kenichiro Shimoji, Yohei Ohno, Toru Egashira, Ruri Kaneda, Mitsushige Murata, Kyoko Hidaka, Takayuki Morisaki, Erika Sasaki, Takeshi Suzuki, Motoaki SanoShinji Makino, Shinzo Oikawa, Keiichi Fukuda

Research output: Contribution to journalArticlepeer-review

289 Citations (Scopus)

Abstract

Several applications of pluripotent stem cell (PSC)-derlved cardlomyocytes require elimination of undifferentiated cells. A major limitation for cardlomyocyte purification Is the lack of easy and specific cell marking techniques. We found that a fluorescent dye that labels mitochondria, tetramethylrhodamlne methyl ester Perchlorate, could be used to selectively mark embryonic and neonatal rat cardlomyocytes, as well as mouse, marmoset and human PSC-derlved cardlomyocytes, and that the cells could subsequently be enriched (>99% purity) by fluorescence-activated cell sorting. Purified cardlomyocytes transplanted Into testes did not Induce teratoma formation. Moreover, aggregate formation of PSC-derlved cardlomyocytes through homophlllc cell-cell adhesion Improved their survival In the lmmunodefident mouse heart. Our approaches will aid In the future success of using PSC-derlved cardlomyocytes for basic and clinical applications.

Original languageEnglish
Pages (from-to)61-66
Number of pages6
JournalNature Methods
Volume7
Issue number1
DOIs
Publication statusPublished - 2010 Jan 1

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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