Nonlinear concentration gradients regulated by the width of channels for observation of half maximal inhibitory concentration (IC<inf>50</inf>) of transporter proteins

Yuta Abe, Koki Kamiya, Toshihisa Osaki, Hirotaka Sasaki, Ryuji Kawano, Norihisa Miki, Shoji Takeuchi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

This paper describes a simple microfluidic device that can generate nonlinear concentration gradients. We changed the "width" of channels that can drastically shorten the total microfluidic channel length and simplify the microfluidic network design rather than the "length" of channels. The logarithmic concentration gradients generated by the device were in good agreement with those obtained by simulation. Using this device, we evaluated a probable IC<inf>50</inf> value of the ABC transporter proteins by the competitive transport assays at five different logarithmic concentrations. This probable IC<inf>50</inf> value was in good agreement with an IC<inf>50</inf> value (0.92 μM) obtained at the diluted concentrations of seven points.

Original languageEnglish
Pages (from-to)5557-5562
Number of pages6
JournalAnalyst
Volume140
Issue number16
DOIs
Publication statusPublished - 2015 Aug 21

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Microfluidics
Inhibitory Concentration 50
Observation
Lab-On-A-Chip Devices
Proteins
protein
Equipment and Supplies
network design
ATP-Binding Cassette Transporters
Protein Transport
Assays
assay
simulation

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy
  • Electrochemistry
  • Biochemistry
  • Environmental Chemistry

Cite this

Nonlinear concentration gradients regulated by the width of channels for observation of half maximal inhibitory concentration (IC<inf>50</inf>) of transporter proteins. / Abe, Yuta; Kamiya, Koki; Osaki, Toshihisa; Sasaki, Hirotaka; Kawano, Ryuji; Miki, Norihisa; Takeuchi, Shoji.

In: Analyst, Vol. 140, No. 16, 21.08.2015, p. 5557-5562.

Research output: Contribution to journalArticle

Abe, Yuta ; Kamiya, Koki ; Osaki, Toshihisa ; Sasaki, Hirotaka ; Kawano, Ryuji ; Miki, Norihisa ; Takeuchi, Shoji. / Nonlinear concentration gradients regulated by the width of channels for observation of half maximal inhibitory concentration (IC<inf>50</inf>) of transporter proteins. In: Analyst. 2015 ; Vol. 140, No. 16. pp. 5557-5562.
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