Normal cadmium uptake in microcytic anemia mk/mk mice suggests that DMT1 is not the only cadmium transporter in vivo

Tomohito Suzuki, Kanae Momoi, Makoto Hosoyamada, Masaki Kimura, Toshiaki Shibasaki

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Divalent metal transporter 1 (DMT1) is a mammalian iron (Fe) transporter and also transports Cadmium (Cd) in vitro. This study compared Cd absorption in DMT1-dysfunctional MK/Rej-mk/mk mice (mk/mk mice) and in DMT1-functional, Fe-deficient wild-type (WT) mice, to clarify the role of DMT1 in intestinal Cd absorption in vivo. Mice were given 1 ppm CdCl2 aq in drinking water for 2 weeks, and the concentrations of Cd and Fe in liver, kidney, and intestinal epithelium were subsequently determined. The Fe concentration in intestinal epithelia of WT mice was decreased in proportion to the level of dietary Fe limitation, while Cd accumulation under the same conditions was increased. DMT1 mRNA expression in the small intestine of Fe-deficient WT mice was clearly increased compared to that in Fe-sufficient WT mice. Iron deficiency resulted in up-regulation of Cd uptake in the intestine of Fe-deficient WT mice. The mk/mk mice have a mutation in DMT1 and loss of its function led to decreased intestinal Fe concentration. However, intestinal Cd accumulation was the same as in WT mice and it was also increased in Fe-deficient situation. There is the possibility that an unknown Cd pathway has taken a role on Cd intestinal absorption in vivo and that this pathway is regulated by food Fe concentrations. Therefore, DMT1 is not the sole transporter of intestinal cadmium absorption in vivo.

Original languageEnglish
Pages (from-to)462-467
Number of pages6
JournalToxicology and Applied Pharmacology
Volume227
Issue number3
DOIs
Publication statusPublished - 2008 Mar 15

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Cadmium
Anemia
Intestinal Absorption
Intestinal Mucosa
Iron
solute carrier family 11- (proton-coupled divalent metal ion transporters), member 2
Cadmium Chloride
Drinking Water
Liver
Small Intestine
Intestines
Up-Regulation
Kidney
Food
Messenger RNA
Mutation

Keywords

  • Cadmium uptake
  • DMT1
  • Intestine
  • Iron deficiency
  • mk/mk mice

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

Cite this

Normal cadmium uptake in microcytic anemia mk/mk mice suggests that DMT1 is not the only cadmium transporter in vivo. / Suzuki, Tomohito; Momoi, Kanae; Hosoyamada, Makoto; Kimura, Masaki; Shibasaki, Toshiaki.

In: Toxicology and Applied Pharmacology, Vol. 227, No. 3, 15.03.2008, p. 462-467.

Research output: Contribution to journalArticle

Suzuki, Tomohito ; Momoi, Kanae ; Hosoyamada, Makoto ; Kimura, Masaki ; Shibasaki, Toshiaki. / Normal cadmium uptake in microcytic anemia mk/mk mice suggests that DMT1 is not the only cadmium transporter in vivo. In: Toxicology and Applied Pharmacology. 2008 ; Vol. 227, No. 3. pp. 462-467.
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