Normal mineralization and nanostructure of sclerotic bone in mice overexpressing Fra-1

P. Roschger, Koichi Matsuo, B. M. Misof, W. Tesch, W. Jochum, E. F. Wagner, P. Fratzl, K. Klaushofer

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22 Citations (Scopus)

Abstract

Increased bone mass due to elevated number of active osteoblasts has been reported for transgenic mice overexpressing the transcription factor Fra-1. To explore the potential of the anabolic action of Fra-1 in treatment of osteoporosis, we examined the integrity of bone matrix generated in Fra-1 transgenic mice. Femora from Fra-1 transgenic (Fra-1 tg) and wild-type littermates were analyzed for bone mineralization density distribution (BMDD) and nanostructure using quantitative backscattered electron imaging (qBEI) and scanning small angle X-ray scattering (scanning-SAXS), respectively. For comparison, we studied mice lacking c-Fos (Fos-/-), which develop osteopetrosis because of the absence of osteoclasts. Morphometrical analysis of metaphyseal spongiosa revealed an up to 5-fold increase in bone volume for Fra-1 transgenic compared to wild type. BMDD indicated a transient lower mineralization of bone for Fra-1 transgenic at 5 and 8 weeks, which became comparable to that of wild-type mice by 8 months. The homogeneity of mineralization was not altered in the Fra-1 transgenic mice at any ages examined. However, it was strikingly reduced in Fos-/- due to an abundance of hypermineralized cartilage. The bone nanostructure did not show abnormalities in Fra-1 transgenic or Fos-/-. These results provide a rationale for the development of therapeutic applications involving Fra-1-induced bone formation.

Original languageEnglish
Pages (from-to)776-782
Number of pages7
JournalBone
Volume34
Issue number5
DOIs
Publication statusPublished - 2004 May

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Keywords

  • Fra-1
  • Sclerotic bone
  • Transgenic

ASJC Scopus subject areas

  • Physiology
  • Hematology

Cite this

Roschger, P., Matsuo, K., Misof, B. M., Tesch, W., Jochum, W., Wagner, E. F., Fratzl, P., & Klaushofer, K. (2004). Normal mineralization and nanostructure of sclerotic bone in mice overexpressing Fra-1. Bone, 34(5), 776-782. https://doi.org/10.1016/j.bone.2004.01.004