Normal ovarian surface epithelial label-retaining cells exhibit stem/progenitor cell characteristics

Paul P. Szotek, Henry L. Chang, Kristen Brennand, Akihiro Fujino, Rafael Pieretti-Vanmarcke, Cristina Lo Celso, David Dombkowski, Frederic Preffer, Kenneth S. Cohen, Jose Teixeira, Patricia K. Donahoe

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

Ovulation induces cyclic rupture and regenerative repair of the ovarian coelomic epithelium. This process of repeated disruption and repair accompanied by complex remodeling typifies a somatic stem/progenitor cell-mediated process. Using BrdU incorporation and doxycycline inducible histone2B-green fluorescent protein pulse-chase techniques, we identify a label-retaining cell population in the coelomic epithelium of the adult mouse ovary as candidate somatic stem/progenitor cells. The identified population exhibits quiescence with asymmetric label retention, functional response to estrous cycling in vivo by proliferation, enhanced growth characteristics by in vitro colony formation, and cytoprotective mechanisms by enrichment for the side population. Together, these characteristics identify the label-retaining cell population as a candidate for the putative somatic stem/progenitor cells of the coelomic epithelium of the mouse ovary.

Original languageEnglish
Pages (from-to)12469-12473
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number34
DOIs
Publication statusPublished - 2008 Aug 26
Externally publishedYes

Fingerprint

Adult Stem Cells
Stem Cells
Epithelium
Population
Ovary
Doxycycline
Bromodeoxyuridine
Ovulation
Green Fluorescent Proteins
Rupture
Growth

Keywords

  • Ovarian coelomic epithelium
  • Somatic stem cells

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Szotek, P. P., Chang, H. L., Brennand, K., Fujino, A., Pieretti-Vanmarcke, R., Celso, C. L., ... Donahoe, P. K. (2008). Normal ovarian surface epithelial label-retaining cells exhibit stem/progenitor cell characteristics. Proceedings of the National Academy of Sciences of the United States of America, 105(34), 12469-12473. https://doi.org/10.1073/pnas.0805012105

Normal ovarian surface epithelial label-retaining cells exhibit stem/progenitor cell characteristics. / Szotek, Paul P.; Chang, Henry L.; Brennand, Kristen; Fujino, Akihiro; Pieretti-Vanmarcke, Rafael; Celso, Cristina Lo; Dombkowski, David; Preffer, Frederic; Cohen, Kenneth S.; Teixeira, Jose; Donahoe, Patricia K.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, No. 34, 26.08.2008, p. 12469-12473.

Research output: Contribution to journalArticle

Szotek, PP, Chang, HL, Brennand, K, Fujino, A, Pieretti-Vanmarcke, R, Celso, CL, Dombkowski, D, Preffer, F, Cohen, KS, Teixeira, J & Donahoe, PK 2008, 'Normal ovarian surface epithelial label-retaining cells exhibit stem/progenitor cell characteristics', Proceedings of the National Academy of Sciences of the United States of America, vol. 105, no. 34, pp. 12469-12473. https://doi.org/10.1073/pnas.0805012105
Szotek, Paul P. ; Chang, Henry L. ; Brennand, Kristen ; Fujino, Akihiro ; Pieretti-Vanmarcke, Rafael ; Celso, Cristina Lo ; Dombkowski, David ; Preffer, Frederic ; Cohen, Kenneth S. ; Teixeira, Jose ; Donahoe, Patricia K. / Normal ovarian surface epithelial label-retaining cells exhibit stem/progenitor cell characteristics. In: Proceedings of the National Academy of Sciences of the United States of America. 2008 ; Vol. 105, No. 34. pp. 12469-12473.
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