Notch ligand mRNA levels of human APCs predict Th1/Th2-promoting activities

Masatoshi Wakui, Kazuhisa Nakano, Sho Matsushita

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Although the contribution of Notch ligands expressed by antigen-presenting cells (APCs) to the Th1/Th2 differentiation has been suggested in mouse studies, their nature in humans remains obscure. To assess the issue, we examined correlation of Notch ligand mRNA levels of human APCs with Th1/Th2-promoting stimulations, i.e. Th1/Th2 adjuvant activities. The expression patterns of human dendritic cells (DCs) and leukemic cell line models of APCs differed partly from those previously observed in mouse DCs and by cellular types and maturation stages. Most strikingly, Th2 adjuvants enhanced the Delta1 expression on human APCs but did not induce Delta4 expression, which was induced by a Th1 adjuvant. The differential expression patterns suggest a distinct role of these Delta members in Th1/Th2 differentiation and their predictive potential for Th1/Th2-promoting activities.

Original languageEnglish
Pages (from-to)596-601
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume358
Issue number2
DOIs
Publication statusPublished - 2007 Jun 29
Externally publishedYes

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Keywords

  • Antigen-presenting cell
  • Delta1
  • Delta4
  • Dendritic cell
  • Leukemic cell line
  • Notch ligand
  • Th1/Th2 adjuvant

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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