Notch1 regulates invasion and metastasis of head and neck squamous cell carcinoma by inducing EMT through c-Myc

Naoya Inamura, Taichi Kimura, Lei Wang, Hiroko Yanagi, Masumi Tsuda, Mishie Tanino, Hiroshi Nishihara, Satoshi Fukuda, Shinya Tanaka

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Objective As 50% of patients of head and neck squamous carcinoma (HNSCC) exhibit poor prognosis, the identification of new therapeutic targets is required. Recently, there have been several reports about the correlation between Notch1 and HNSCC, but the precise mechanism is still obscure. Therefore, in this study, we examined the involvement of Notch1 in HNSCC by using HNSCC cell lines and surgical specimens. Methods To investigate the role of Notch1 in HNSCC, we examined the effect of Notch inhibitor DAPT on cell growth, invasion, and tumorigenicity using five HNSCC cell lines in vitro and in vivo. We further examined that the correlation with Notch expression and clinical prognostic factors was evaluated by using 101 HNSCC surgical specimens. Results DAPT reduced the nuclear expression of Notch and c-Myc and repressed cell growth, EMT-dependent cell invasion in vitro, and tumorigenicity in vivo. An overexpression of Myc enhanced EMT with an increase of Snail and vimentin together with decreased levels of E-cadherin in HSC3 cells. Finally, we discovered that Notch expression was well correlated with MIB-1 index and lymph node metastases. Conclusion We discovered that Notch1 was strongly correlated with HNSCC growth, invasion, and metastases. Therefore, Notch1 might be a new therapeutic target and a predictive marker of proliferation and metastasis of HNSCC.

Original languageEnglish
Pages (from-to)447-457
Number of pages11
JournalAuris Nasus Larynx
Volume44
Issue number4
DOIs
Publication statusPublished - 2017 Aug 1
Externally publishedYes

Fingerprint

Squamous Cell Carcinoma
Neck
Head
Neoplasm Metastasis
Growth
Carcinoma, squamous cell of head and neck
Cell Line
Vimentin
Cadherins
Lymph Nodes
Therapeutics

Keywords

  • Growth
  • Head and neck cancer
  • Invasion
  • Notch1
  • Squamous cell carcinoma

ASJC Scopus subject areas

  • Surgery
  • Otorhinolaryngology

Cite this

Notch1 regulates invasion and metastasis of head and neck squamous cell carcinoma by inducing EMT through c-Myc. / Inamura, Naoya; Kimura, Taichi; Wang, Lei; Yanagi, Hiroko; Tsuda, Masumi; Tanino, Mishie; Nishihara, Hiroshi; Fukuda, Satoshi; Tanaka, Shinya.

In: Auris Nasus Larynx, Vol. 44, No. 4, 01.08.2017, p. 447-457.

Research output: Contribution to journalArticle

Inamura, N, Kimura, T, Wang, L, Yanagi, H, Tsuda, M, Tanino, M, Nishihara, H, Fukuda, S & Tanaka, S 2017, 'Notch1 regulates invasion and metastasis of head and neck squamous cell carcinoma by inducing EMT through c-Myc', Auris Nasus Larynx, vol. 44, no. 4, pp. 447-457. https://doi.org/10.1016/j.anl.2016.08.003
Inamura, Naoya ; Kimura, Taichi ; Wang, Lei ; Yanagi, Hiroko ; Tsuda, Masumi ; Tanino, Mishie ; Nishihara, Hiroshi ; Fukuda, Satoshi ; Tanaka, Shinya. / Notch1 regulates invasion and metastasis of head and neck squamous cell carcinoma by inducing EMT through c-Myc. In: Auris Nasus Larynx. 2017 ; Vol. 44, No. 4. pp. 447-457.
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AU - Tsuda, Masumi

AU - Tanino, Mishie

AU - Nishihara, Hiroshi

AU - Fukuda, Satoshi

AU - Tanaka, Shinya

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N2 - Objective As 50% of patients of head and neck squamous carcinoma (HNSCC) exhibit poor prognosis, the identification of new therapeutic targets is required. Recently, there have been several reports about the correlation between Notch1 and HNSCC, but the precise mechanism is still obscure. Therefore, in this study, we examined the involvement of Notch1 in HNSCC by using HNSCC cell lines and surgical specimens. Methods To investigate the role of Notch1 in HNSCC, we examined the effect of Notch inhibitor DAPT on cell growth, invasion, and tumorigenicity using five HNSCC cell lines in vitro and in vivo. We further examined that the correlation with Notch expression and clinical prognostic factors was evaluated by using 101 HNSCC surgical specimens. Results DAPT reduced the nuclear expression of Notch and c-Myc and repressed cell growth, EMT-dependent cell invasion in vitro, and tumorigenicity in vivo. An overexpression of Myc enhanced EMT with an increase of Snail and vimentin together with decreased levels of E-cadherin in HSC3 cells. Finally, we discovered that Notch expression was well correlated with MIB-1 index and lymph node metastases. Conclusion We discovered that Notch1 was strongly correlated with HNSCC growth, invasion, and metastases. Therefore, Notch1 might be a new therapeutic target and a predictive marker of proliferation and metastasis of HNSCC.

AB - Objective As 50% of patients of head and neck squamous carcinoma (HNSCC) exhibit poor prognosis, the identification of new therapeutic targets is required. Recently, there have been several reports about the correlation between Notch1 and HNSCC, but the precise mechanism is still obscure. Therefore, in this study, we examined the involvement of Notch1 in HNSCC by using HNSCC cell lines and surgical specimens. Methods To investigate the role of Notch1 in HNSCC, we examined the effect of Notch inhibitor DAPT on cell growth, invasion, and tumorigenicity using five HNSCC cell lines in vitro and in vivo. We further examined that the correlation with Notch expression and clinical prognostic factors was evaluated by using 101 HNSCC surgical specimens. Results DAPT reduced the nuclear expression of Notch and c-Myc and repressed cell growth, EMT-dependent cell invasion in vitro, and tumorigenicity in vivo. An overexpression of Myc enhanced EMT with an increase of Snail and vimentin together with decreased levels of E-cadherin in HSC3 cells. Finally, we discovered that Notch expression was well correlated with MIB-1 index and lymph node metastases. Conclusion We discovered that Notch1 was strongly correlated with HNSCC growth, invasion, and metastases. Therefore, Notch1 might be a new therapeutic target and a predictive marker of proliferation and metastasis of HNSCC.

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