Novel application of proteinchip technology exploring acute rejection markers of rat small bowel transplantation

Yasuko Yamayoshi, Toshihiko Watanabe, Minoru Tanabe, Ken Hoshino, Koshi Matsumoto, Yasuhide Morikawa, Motohide Shimadzu, Masaki Kitajima, Yusuke Tanigawara

Research output: Contribution to journalArticle

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Abstract

BACKGROUND. Because no biomarker that reflects small bowel allograft rejection is available, we applied surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) to develop noninvasive markers required for routine diagnosis. METHODS. Heterotopic small bowel transplantation (SBT) was performed in rats, and they were divided into four experimental groups: sham-operated rats (sham), syngeneic transplants (syngeneic), allogeneic transplants (allogeneic), allogeneic transplants received FK506 (allo+FK). Plasma samples were analyzed with SELDI ProteinChip arrays to detect peaks that predominated in the allogeneic model. Possible biomarkers were identified in combination with SELDI retentate chromatography mass spectrometry (RCMS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). The identified protein was further analyzed by immunohistochemistry. RESULTS. An increase in the level of a 14.8-kDa protein, identified as lysozyme, was observed specifically in the plasma of the allogeneic group; the levels of this protein remained unchanged in the plasma of the other groups. On the other hand, the levels of a 10.1-kDa and a 13.0-kDa protein, identified as migration inhibitory factor-related proteins (MRP), MRP-8 and MRP-14, respectively, began to increase from an early stage of acute rejection. We also observed that lysozyme-positive macrophages had strongly infiltrated the lamina propria during acute rejection. CONCLUSIONS. We identified three plasma proteins-MRP-8, MRP-14, and lysozyme-that increased during small bowel allograft rejection. The identified proteins appeared to be markers for inflammation associated with allograft rejection. This proteomic approach will be useful for the identification of candidate biomarkers.

Original languageEnglish
Pages (from-to)320-326
Number of pages7
JournalTransplantation
Volume82
Issue number3
DOIs
Publication statusPublished - 2006

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Protein Array Analysis
Transplantation
Technology
Transplants
Muramidase
Allografts
Proteins
Biomarkers
Mass Spectrometry
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Tacrolimus
Proteomics
Chromatography
Blood Proteins
Mucous Membrane
Lasers
Immunohistochemistry
Macrophages
Inflammation

Keywords

  • Acute rejection
  • Biomarker
  • Lysozyme
  • SELDI-TOF MS
  • Small bowel transplantation

ASJC Scopus subject areas

  • Transplantation
  • Immunology

Cite this

Novel application of proteinchip technology exploring acute rejection markers of rat small bowel transplantation. / Yamayoshi, Yasuko; Watanabe, Toshihiko; Tanabe, Minoru; Hoshino, Ken; Matsumoto, Koshi; Morikawa, Yasuhide; Shimadzu, Motohide; Kitajima, Masaki; Tanigawara, Yusuke.

In: Transplantation, Vol. 82, No. 3, 2006, p. 320-326.

Research output: Contribution to journalArticle

Yamayoshi, Y, Watanabe, T, Tanabe, M, Hoshino, K, Matsumoto, K, Morikawa, Y, Shimadzu, M, Kitajima, M & Tanigawara, Y 2006, 'Novel application of proteinchip technology exploring acute rejection markers of rat small bowel transplantation', Transplantation, vol. 82, no. 3, pp. 320-326. https://doi.org/10.1097/01.tp.0000228909.49640.08
Yamayoshi, Yasuko ; Watanabe, Toshihiko ; Tanabe, Minoru ; Hoshino, Ken ; Matsumoto, Koshi ; Morikawa, Yasuhide ; Shimadzu, Motohide ; Kitajima, Masaki ; Tanigawara, Yusuke. / Novel application of proteinchip technology exploring acute rejection markers of rat small bowel transplantation. In: Transplantation. 2006 ; Vol. 82, No. 3. pp. 320-326.
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AU - Yamayoshi, Yasuko

AU - Watanabe, Toshihiko

AU - Tanabe, Minoru

AU - Hoshino, Ken

AU - Matsumoto, Koshi

AU - Morikawa, Yasuhide

AU - Shimadzu, Motohide

AU - Kitajima, Masaki

AU - Tanigawara, Yusuke

PY - 2006

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N2 - BACKGROUND. Because no biomarker that reflects small bowel allograft rejection is available, we applied surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) to develop noninvasive markers required for routine diagnosis. METHODS. Heterotopic small bowel transplantation (SBT) was performed in rats, and they were divided into four experimental groups: sham-operated rats (sham), syngeneic transplants (syngeneic), allogeneic transplants (allogeneic), allogeneic transplants received FK506 (allo+FK). Plasma samples were analyzed with SELDI ProteinChip arrays to detect peaks that predominated in the allogeneic model. Possible biomarkers were identified in combination with SELDI retentate chromatography mass spectrometry (RCMS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). The identified protein was further analyzed by immunohistochemistry. RESULTS. An increase in the level of a 14.8-kDa protein, identified as lysozyme, was observed specifically in the plasma of the allogeneic group; the levels of this protein remained unchanged in the plasma of the other groups. On the other hand, the levels of a 10.1-kDa and a 13.0-kDa protein, identified as migration inhibitory factor-related proteins (MRP), MRP-8 and MRP-14, respectively, began to increase from an early stage of acute rejection. We also observed that lysozyme-positive macrophages had strongly infiltrated the lamina propria during acute rejection. CONCLUSIONS. We identified three plasma proteins-MRP-8, MRP-14, and lysozyme-that increased during small bowel allograft rejection. The identified proteins appeared to be markers for inflammation associated with allograft rejection. This proteomic approach will be useful for the identification of candidate biomarkers.

AB - BACKGROUND. Because no biomarker that reflects small bowel allograft rejection is available, we applied surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) to develop noninvasive markers required for routine diagnosis. METHODS. Heterotopic small bowel transplantation (SBT) was performed in rats, and they were divided into four experimental groups: sham-operated rats (sham), syngeneic transplants (syngeneic), allogeneic transplants (allogeneic), allogeneic transplants received FK506 (allo+FK). Plasma samples were analyzed with SELDI ProteinChip arrays to detect peaks that predominated in the allogeneic model. Possible biomarkers were identified in combination with SELDI retentate chromatography mass spectrometry (RCMS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). The identified protein was further analyzed by immunohistochemistry. RESULTS. An increase in the level of a 14.8-kDa protein, identified as lysozyme, was observed specifically in the plasma of the allogeneic group; the levels of this protein remained unchanged in the plasma of the other groups. On the other hand, the levels of a 10.1-kDa and a 13.0-kDa protein, identified as migration inhibitory factor-related proteins (MRP), MRP-8 and MRP-14, respectively, began to increase from an early stage of acute rejection. We also observed that lysozyme-positive macrophages had strongly infiltrated the lamina propria during acute rejection. CONCLUSIONS. We identified three plasma proteins-MRP-8, MRP-14, and lysozyme-that increased during small bowel allograft rejection. The identified proteins appeared to be markers for inflammation associated with allograft rejection. This proteomic approach will be useful for the identification of candidate biomarkers.

KW - Acute rejection

KW - Biomarker

KW - Lysozyme

KW - SELDI-TOF MS

KW - Small bowel transplantation

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